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Precision-cut lung slices: A powerful ex vivo model to investigate respiratory infectious diseases
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-09-27 , DOI: 10.1111/mmi.14817
Flávia Viana 1 , Cecilia M O'Kane 1 , Gunnar N Schroeder 1
Affiliation  

Respiratory infections are a leading cause of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid, or surrogate animal models. Although these provide important insights, they have limitations. Cell culture models fail to recapitulate cellular interactions in the lung and animal models often do not permit high-throughput analysis of drugs or pathogen isolates; hence, there is a need for improved, scalable models. Precision-cut lung slices (PCLS), small, uniform tissue slices generated from animal or human lungs are increasingly recognized and employed as an ex vivo organotypic model. PCLS retain remarkable cellular complexity and the architecture of the lung, providing a platform to investigate respiratory pathogens in a near-native environment. Here, we review the generation and features of PCLS, their use to investigate the pathogenesis of viral and bacterial pathogens, and highlight their potential to advance respiratory infection research in the future.

中文翻译:

精密切割肺切片:研究呼吸道传染病的强大离体模型

呼吸道感染是全球死亡的主要原因。大多数关于潜在疾病机制的研究都是基于细胞培养、类器官或替代动物模型。尽管这些提供了重要的见解,但它们有局限性。细胞培养模型无法概括肺中的细胞相互作用,动物模型通常不允许对药物或病原体分离物进行高通量分析;因此,需要改进的、可扩展的模型。精确切割的肺切片 (PCLS),由动物或人肺产生的小而均匀的组织切片,越来越多地被认可并用作离体器官模型。PCLS 保留了显着的细胞复杂性和肺的结构,为在近乎原生的环境中研究呼吸道病原体提供了一个平台。这里,
更新日期:2021-09-27
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