Insulin has metabolic and vascular effects in the human body. What mechanisms that orchestrate the effects in the microcirculation, and how the responds differ in different tissues, is however not fully understood. It is therefore of interest to search for markers in microdialysate that may be related to the microcirculation. This study aims to identify proteins related to microvascular changes in different tissue compartments after glucose provocation using in vivo microdialysis. Microdialysis was conducted in three different tissue compartments (intracutaneous, subcutaneous and intravenous) from healthy subjects. Microdialysate was collected during three time periods; recovery after catheter insertion, baseline and glucose provocation, and analyzed using proteomics. Altogether, 126 proteins were detected. Multivariate data analysis showed that the differences in protein expression levels during the three time periods, including comparison before and after glucose provocation, were most pronounced in the intracutaneous and subcutaneous compartments. Four proteins with vascular effects were identified (angiotensinogen, kininogen-1, alpha-2-HS-glycoprotein and hemoglobin subunit beta), all upregulated after glucose provocation compared to baseline in all three compartments. Glucose provocation is known to cause insulin-induced vasodilation through the nitric oxide pathway, and this study indicates that this is facilitated through the interactions of the RAS (angiotensinogen) and kallikrein-kinin (kininogen-1) systems.

胰岛素在人体内具有代谢和血管作用。然而，在微循环中协调作用的机制是什么，以及不同组织的反应如何不同，尚不完全清楚。因此，在微透析液中寻找可能与微循环有关的标志物是很有意义的。本研究旨在使用体内微透析来鉴定与葡萄糖激发后不同组织隔室中微血管变化相关的蛋白质。微透析在来自健康受试者的三个不同组织隔室（皮内、皮下和静脉内）中进行。在三个时间段内收集微透析液；导管插入、基线和葡萄糖激发后的恢复情况，并使用蛋白质组学进行分析。总共检测到 126 种蛋白质。多变量数据分析表明，三个时间段内蛋白质表达水平的差异，包括葡萄糖激发前后的比较，在皮内和皮下区室中最为明显。确定了四种具有血管作用的蛋白质（血管紧张素原、激肽原-1、α-2-HS-糖蛋白和血红蛋白亚基β），与所有三个隔室的基线相比，在葡萄糖激发后均上调。已知葡萄糖激发通过一氧化氮途径引起胰岛素诱导的血管舒张，这项研究表明这是通过 RAS（血管紧张素原）和激肽释放酶激肽（激肽原-1）系统的相互作用促进的。在皮内和皮下隔室中最为明显。确定了四种具有血管作用的蛋白质（血管紧张素原、激肽原-1、α-2-HS-糖蛋白和血红蛋白亚基β），与所有三个隔室的基线相比，在葡萄糖激发后均上调。已知葡萄糖激发通过一氧化氮途径引起胰岛素诱导的血管舒张，这项研究表明这是通过 RAS（血管紧张素原）和激肽释放酶激肽（激肽原-1）系统的相互作用促进的。在皮内和皮下隔室中最为明显。确定了四种具有血管作用的蛋白质（血管紧张素原、激肽原-1、α-2-HS-糖蛋白和血红蛋白亚基β），与所有三个隔室的基线相比，在葡萄糖激发后均上调。已知葡萄糖激发通过一氧化氮途径引起胰岛素诱导的血管舒张，这项研究表明这是通过 RAS（血管紧张素原）和激肽释放酶激肽（激肽原-1）系统的相互作用促进的。