Biosynthesized nanoparticles have played vital role recently, as suggested to be alternative to physical and chemical methods. In this study, biosynthesis of zinc oxide nanoparticles (ZnO NPs) were carried out using leaf extracts of Phoenix dactylifera L. and Zinc nitrate. The effect of ZnO nanoparticles on biomass and biochemical parameters was investigated. Biosynthesized ZnO nanostructure was characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), UV–visible spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Which resulted in spherical shape with size ranging between 16 to 35 nm of Biosynthesized ZnO nanoparticles and UV absorption beak at 370.5 nm with clear peaks of functional groups. The impact of different concentrations (0.0 mg/L, 80 mg/L and 160 mg/L) of biosynthesized ZnO nanoparticles on biomass and bioactive compounds production of Juniperus procera in vitro was investigated. The results showed that, biosynthesized ZnO NPs (80 mg/L and 160 mg/L) concentrations were boosted the growth of J. Procera with significantly compared to non-treated plants in vitro. The highest concentration (160 mg/L) of ZnO NPs was enhanced the growth of plant at beginning period, one month later shoots became yellow and callus turned to be brownish. Moreover, the influence of ZnO NPs on phytochemical compounds in callus of Juniperus procera was examined using GC–MS analysis. The differences among treatments were recoded. Overall, zinc oxide nanoparticles substantially improved the growth of shoots and callus with increasing of biochemical parameters such as chlorophyll a, total phenolic and flavonoids contents, besides the total protein and, SOD, CAT and APX activity. ZnO NPs might be induced some phytochemical compounds as well as inhibit.

生物合成的纳米粒子最近发挥了重要作用，被建议替代物理和化学方法。在这项研究中，使用Phoenix dactylifera L的叶提取物进行了氧化锌纳米颗粒 (ZnO NPs) 的生物合成。和硝酸锌。研究了 ZnO 纳米粒子对生物量和生化参数的影响。使用 X 射线衍射 (XRD)、透射电子显微镜 (TEM)、紫外-可见分光光度计和傅里叶变换红外光谱 (FTIR) 表征生物合成的 ZnO 纳米结构。这导致生物合成的 ZnO 纳米粒子的尺寸范围在 16 至 35 nm 之间，并且在 370.5 nm 处有紫外吸收峰，具有清晰的官能团峰。研究了不同浓度（0.0 mg/L、80 mg/L 和 160 mg/L）生物合成 ZnO 纳米粒子对刺柏体外生物量和生物活性化合物产量的影响。结果表明，生物合成的 ZnO NPs（80 mg/L 和 160 mg/L）浓度促进了J. Procera在体外与未处理的植物相比具有显着性。最高浓度（160 mg/L）的ZnO NPs在初期促进了植物的生长，一个月后芽变黄，愈伤组织变成褐色。此外，使用 GC-MS 分析法研究了 ZnO NPs 对刺柏愈伤组织中植物化学成分的影响。记录处理之间的差异。总体而言，氧化锌纳米颗粒显着改善了芽和愈伤组织的生长，增加了生化参数，如叶绿素 a、总酚和类黄酮含量，以及总蛋白质和 SOD、CAT 和 APX 活性。ZnO NPs 可能会诱导一些植物化学化合物以及抑制。