The purpose of this study was to elucidate, under which conditions abattoir-acquired pig eyes are suitable for refractive excimer laser experiments. Porcine eyes from tunnel-scalded (n = 5) and tank-scalded (n = 10) pigs were compared to unscalded eyes (n = 5) and to eyes scalded in the laboratory (n = 5). The corneal epithelium was removed before an excimer laser was used to perform a − 8.0 D photoablation. Corneal thickness was measured by optical coherence topography before and after photoablation. The ablation depth was determined with a contour measuring station, the morphology of the ablated areas was characterized by scanning electron microscopy and white-light profilometry. The scalded eyes showed an increase in corneal swelling which gained statistical significance in tank-scalded eyes showing a wedge-shaped opaque stromal lesion in the nasal corneal quadrant. A measurable deterioration of photoablation was only found in tank-scalded eyes that exhibited the opaque lesion. Ablated area morphology was smooth and regular in the unscalded and tunnel-scalded eyes. The tank-scalded eyes showed conspicuous wrinkles. While unscalded eyes should always be preferred for excimer laser laboratory experiments, the data suggest that the use of tunnel-scalded eyes may also be acceptable and should be chosen over tank-scalded eyes.

本研究的目的是阐明屠宰场获得的猪眼适用于屈光准分子激光实验的条件。将隧道烫伤 (n = 5) 和水槽烫伤 (n = 10) 猪的猪眼与未烫伤的眼睛 (n = 5) 和实验室烫伤的眼睛 (n = 5) 进行比较。在使用准分子激光进行 - 8.0 D 光消融之前去除角膜上皮。在光消融前后通过光学相干形貌测量角膜厚度。消融深度由轮廓测量站确定，消融区域的形态通过扫描电子显微镜和白光轮廓仪进行表征。烫伤的眼睛显示角膜肿胀增加，这在水箱烫伤的眼睛中获得了统计学显着性，显示鼻角膜象限中的楔形不透明基质病变。仅在呈现不透明病变的水槽烫伤眼睛中发现了可测量的光消融恶化。在未烫伤和隧道烫伤的眼睛中，烧蚀区域形态光滑且规则。被水箱烫过的眼睛，出现了明显的皱纹。虽然准分子激光实验室实验应始终首选未烫伤的眼睛，但数据表明，使用隧道烫伤的眼睛也可能是可以接受的，应选择而不是水箱烫伤的眼睛。在未烫伤和隧道烫伤的眼睛中，烧蚀区域形态光滑且规则。被水箱烫过的眼睛，出现了明显的皱纹。虽然准分子激光实验室实验应始终首选未烫伤的眼睛，但数据表明，使用隧道烫伤的眼睛也可能是可以接受的，应选择而不是水箱烫伤的眼睛。在未烫伤和隧道烫伤的眼睛中，烧蚀区域形态光滑且规则。被水箱烫过的眼睛，出现了明显的皱纹。虽然准分子激光实验室实验应始终首选未烫伤的眼睛，但数据表明，使用隧道烫伤的眼睛也可能是可以接受的，应选择而不是水箱烫伤的眼睛。