Prunus mume blossom is an edible flower that has been used in traditional Chinese medicine for thousands of years. Flavonoids are one of the most active substances in Prunus mume blossoms. The optimal ultrasonic-assisted enzymatic extraction of flavonoids from Prunus mume blossom (FPMB), the components of FPMB, and its protective effect on injured cardiomyocytes were investigated in this study. According to our results, the optimal extraction process for FPMB is as follows: cellulase at 2.0%, ultrasonic power at 300 W, ultrasonic enzymolysis for 30 min, and an enzymolysis temperature of 40 °C. FPMB significantly promoted the survival rate of cardiomyocytes and reduced the concentration of reactive oxygen species (ROS). FPMB also improved the activities of proteases caspase-3, caspase-8, and caspase-9 in cardiomyocytes. The cardiomyocyte apoptosis rate in mice was significantly reduced by exposure to FPMB. These results suggest that the extraction rate of FPMB may be improved by an ultrasonic-assisted enzymatic method. FPMB has a protective effect on the injured cardiomyocytes.

中文翻译：

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梅花黄酮超声辅助酶解提取及对小鼠损伤心肌细胞的保护作用

梅花是一种可食用的花卉，在传统中药中已使用了数千年。黄酮类化合物是梅花中最活跃的物质之一。梅花中黄酮类化合物的最佳超声辅助酶法提取本研究调查了开花 (FPMB)、FPMB 的成分及其对受损心肌细胞的保护作用。根据我们的结果，FPMB 的最佳提取工艺如下：纤维素酶为 2.0%，超声功率为 300 W，超声酶解 30 分钟，酶解温度为 40 °C。FPMB显着提高了心肌细胞的存活率并降低了活性氧（ROS）的浓度。FPMB 还提高了心肌细胞中蛋白酶 caspase-3、caspase-8 和 caspase-9 的活性。暴露于FPMB后，小鼠的心肌细胞凋亡率显着降低。这些结果表明超声波辅助酶法可以提高 FPMB 的提取率。FPMB 对受损的心肌细胞有保护作用。