Optical coherence microscopy is an imaging technique capable of generating three-dimensional images of structures in tissue, but with limited functional imaging ability. Fluorescence microscopy offers functional information of cellular activities. The integration of both imaging modalities can enable the evaluation of the impact of structural information on cells activities. To develop an integrated system combining high-resolution optical coherence microscopy (HR-OCM) and dual-channel scanning confocal fluorescence microscopy (DC-SCFM) to enable the co-registration of structural and functional information. Different strategies to enable the simultaneous recording of information, as well as to overcome the focal plane mismatch between both imaging modalities were developed. The system's performances were evaluated in imaging fluorescence microspheres embedded in multi-layer tape and silicone phantom. HR-OCM achieved an axial resolution of 2.4-µm in tissue over an imaging depth of 1-mm. A speed of 250 kHz and a lateral resolution of 2-µm over a field-of-view of 1.1 mm ×1.1 mm were demonstrated with the combined system. DC-SCFM allowed to discriminate between green and red microspheres while HR-OCM enabled their depth localization. The combined system is synergistic in generating structural and functional information of samples, and has the potential to enable new investigations of biological processes in animal models.

光学相干显微镜是一种成像技术，能够生成组织中结构的三维图像，但功能成像能力有限。荧光显微镜提供细胞活动的功能信息。两种成像方式的集成可以评估结构信息对细胞活动的影响。开发结合高分辨率光学相干显微镜 (HR-OCM) 和双通道扫描共聚焦荧光显微镜 (DC-SCFM) 的集成系统，以实现结构和功能信息的共同注册。开发了不同的策略来同时记录信息，以及克服两种成像模式之间的焦平面不匹配。系统' 在嵌入多层胶带和硅胶假体中的成像荧光微球中评估了 s 性能。HR-OCM 在 1 毫米的成像深度内在组织中实现了 2.4 微米的轴向分辨率。该组合系统在 1.1 毫米 × 1.1 毫米的视场内展示了 250 kHz 的速度和 2 µm 的横向分辨率。DC-SCFM 允许区分绿色和红色微球，而 HR-OCM 启用它们的深度定位。该组合系统在生成样本的结构和功能信息方面具有协同作用，并有可能在动物模型中对生物过程进行新的研究。该组合系统在 1.1 毫米 × 1.1 毫米的视场内展示了 250 kHz 的速度和 2 µm 的横向分辨率。DC-SCFM 允许区分绿色和红色微球，而 HR-OCM 启用它们的深度定位。该组合系统在生成样本的结构和功能信息方面具有协同作用，并有可能在动物模型中对生物过程进行新的研究。该组合系统在 1.1 毫米 × 1.1 毫米的视场内展示了 250 kHz 的速度和 2 µm 的横向分辨率。DC-SCFM 允许区分绿色和红色微球，而 HR-OCM 启用它们的深度定位。该组合系统在生成样本的结构和功能信息方面具有协同作用，并有可能在动物模型中对生物过程进行新的研究。