With the advantages of simple equipment and in-situ preparation of nanopores, the CBD method has been widely concerned by researchers. In this work, a fluorescence platform was set up to directly observe the λ-DNA motions near the CBD prepared different kinds of SiN_x nanopores. At first, for the one nanopore film, the λ-DNA post-translocation motion was observed. Then, for the multiple nanopores film, the current modulation phenomena of each nanopore after the DNA clogged the pores was observed. When the distance of asymmetric pores is less than the length of λ-DNA, the larger nanopores are easy to be blocked preferentially by considering the tug-of-war on λ-DNA; when the asymmetric porous distance is larger than the DNA two polymer length, due to the forming a folded structure, the smaller nanopores are preferentially blocked. These results reveal that the clogging phenomena during the DNA translocation analysis for the nanopores are varied, and it should be considered for CBD-prepared nanopores.

CBD法以其设备简单、纳米孔原位制备等优点受到研究人员的广泛关注。在这项工作中，建立了一个荧光平台来直接观察 CBD 附近的 λ-DNA 运动制备的不同种类的 SiN _x纳米孔。首先，对于一个纳米孔膜，观察到 λ-DNA 后易位运动。然后，对于多纳米孔薄膜，观察DNA堵塞孔后每个纳米孔的电流调节现象。当不对称孔的距离小于λ-DNA的长度时，考虑到对λ-DNA的拉锯战，较大的纳米孔容易被优先阻塞；当不对称孔距大于 DNA 两个聚合物长度时，由于形成折叠结构，较小的纳米孔优先被阻塞。这些结果表明，纳米孔 DNA 易位分析过程中的堵塞现象是多种多样的，对于 CBD 制备的纳米孔应该考虑。