Biochemical, antioxidant, serum, and urine profiles together with physical examination can deliver important information regarding animal health status, and are vital in the diagnosis and treatment of patients. CCl₄, a potent nephrotoxin, was used for causing toxicity in rat kidneys. The present study aimed at exploring the nephroprotective potential of P. jacquemontiana leaves methanol extract (PJM) and P. hydaspidis whole-plant methanol extract (PHM) on kidney cells of male rats after oxidative stress and DNA damage was instigated by CCl₄. Various parameters including enzymatic levels, serum profiles, urine profiles, genotoxicity, and histological studies were conducted. In renal samples of rats treated with CCl₄, the antioxidant enzymes (POD, SOD, CAT), PH level, protein level, and glutathione contents were significantly (p < 0.05) declined whereas renal biochemicals (H₂O₂, TBARS, and nitrite), specific gravity, level of urea, urobilinogen, serum BUN and creatinine were markedly (p < 0.05) increased relative to control group. Co-administration of PJM and PHM with CCl₄ displayed protective ability against CCl₄ intoxication by restoring activities of antioxidant enzymes, urine profile, biochemical parameters, and serum profile in rats. CCl₄ also induced prominent DNA damages and glomerular atrophy with abnormal appearance of glomerulus and Bowman’s capsule. These damages results in impaired corticular sections, edema in Bowman’s capsule, accumulation of necrotic cells, dilation of convoluted tubules, and narrowing of space between Bowman’s capsule, which were successfully ameliorated after co-administration of PJM and PHM fractions in a dose-dependent manner (200 and 400 mg/kg b.w.). The results obtained suggest the therapeutic role of PJM and PHM in oxidative-stress related disorders of kidney and may be helpful in kidney trauma.

生化、抗氧化剂、血清和尿液分析以及体格检查可以提供有关动物健康状况的重要信息，对患者的诊断和治疗至关重要。CCl ₄是一种强效肾毒素，用于引起大鼠肾脏毒性。本研究旨在探讨黄杨叶甲醇提取物（PJM）和紫花杨全株甲醇提取物（PHM）对氧化应激和CCl ₄引起的DNA损伤后雄性大鼠肾细胞的肾保护作用。进行了各种参数，包括酶水平、血清特征、尿液特征、遗传毒性和组织学研究。在用 CCl _{4处理的大鼠的肾脏样本中}，抗氧化酶（POD、SOD、CAT）、PH 水平、蛋白质水平和谷胱甘肽含量显着下降（p < 0.05），而肾脏生化指标（H ₂ O ₂、TBARS 和亚硝酸盐）、比重、尿素水平, 尿胆原、血清 BUN 和肌酐相对于对照组显着增加 (p < 0.05)。_{PJM 和 PHM 与 CCl 4}的共同给药通过恢复大鼠的抗氧化酶活性、尿液特征、生化参数和血清特征，显示出对 CCl _{4中毒的保护能力。}四氯化碳₄还引起显着的 DNA 损伤和肾小球萎缩，肾小球和鲍曼氏囊外观异常。这些损伤导致皮质部分受损、Bowman 囊水肿、坏死细胞积聚、曲小管扩张和 Bowman 囊之间的空间变窄，在以剂量依赖性方式共同施用 PJM 和 PHM 部分后成功改善（200 和 400 毫克/公斤体重）。获得的结果表明 PJM 和 PHM 在肾脏氧化应激相关疾病中的治疗作用，并且可能有助于肾脏创伤。