Hepatitis E virus (HEV) can infect humans, pigs, and many other animals, but recombination in HEV has rarely been reported. In the present study, phylogenetic and recombination analysis was performed on 557 complete HEV genome sequences from the GenBank database. A potentially significant quadruple recombination event was identified by recombination detection analysis. The recombinant progeny virus, HEV_32_Manchester_301214, was produced by inter-genotype recombination between the major parent HEPAC-44 and the minor parent HE-JA15-1335. HEV_32_Manchester_301214 and HEPAC-44 belong to genotype 3, while HE-JA15-1335 belongs to genotype 1, and these three strains were all isolated from humans. Three breakpoints of the four recombination events occurred in the ORF2 region, while another occurred in the ORF1 region. This quadruple recombination event was confirmed by phylogenetic analysis. The genotype, host, and recombination regions of the three strains were analyzed, and the analysis results provide valuable information for future research on HEV diversity.

戊型肝炎病毒 (HEV) 可以感染人类、猪和许多其他动物，但很少报道 HEV 中的重组。在本研究中，对来自 GenBank 数据库的 557 个完整的 HEV 基因组序列进行了系统发育和重组分析。通过重组检测分析确定了潜在的重要四重重组事件。重组子代病毒 HEV_32_Manchester_301214 是通过主要亲本 HEPAC-44 和次要亲本 HE-JA15-1335 之间的基因型间重组产生的。HEV_32_Manchester_301214 和 HEPAC-44 属于基因 3 型，而 HE-JA15-1335 属于基因 1 型，这三个毒株均分离自人类。四个重组事件的三个​​断点发生在 ORF2 区域，而另一个发生在 ORF1 区域。通过系统发育分析证实了这种四重重组事件。对3个菌株的基因型、宿主和重组区进行了分析，分析结果为未来HEV多样性研究提供了有价值的信息。