Genome editing (also known as gene editing) employs a range of tools such as Meganucleases, Zinc Finger Nucleases, TALENs, and more recently CRISPR to make defined changes in genes, regulatory sequences, untranslated regions, or intergenic regions. It is increasingly being applied in plant science research and to improve plant varieties. The benefits of having effective detection tools begin with optimization of the genome editing process itself and continue with selection and characterization of tissue cultures and/or regenerated plants. Detection tools are also used throughout the breeding process, and for preparation of regulatory dossiers when required, as well as for seed production, and may be necessary for monitoring products in the marketplace. Detection and identification of genome edits employs a wide range of analytical approaches including PCR, digital PCR, and sequencing methods. This article examines the applicability of each category of detection or identification approach, from the optimization of genome editing processes, through creation of edits, selection and characterization, and breeding. The challenges surrounding the detection of genome edits present at low levels in large seed, plant, or grain populations and of differentiating directed genome edits from conventional mutations are also explained.

基因组编辑（也称为基因编辑）使用一系列工具，例如大范围核酸酶、锌指核酸酶、TALEN 和最近的 CRISPR，对基因、调控序列、非翻译区或基因间区域进行明确的更改。它越来越多地应用于植物科学研究和改良植物品种。拥有有效检测工具的好处始于优化基因组编辑过程本身，并继续选择和表征组织培养物和/或再生植物。检测工具还用于整个育种过程，并在需要时用于准备监管档案以及种子生产，并且可能是监测市场产品所必需的。基因组编辑的检测和鉴定采用多种分析方法，包括 PCR、数字 PCR 和测序方法。本文从基因组编辑过程的优化，到编辑的创建、选择和表征以及育种，研究了每一类检测或识别方法的适用性。还解释了围绕检测大种子、植物或谷物种群中低水平的基因组编辑以及区分定向基因组编辑与常规突变的挑战​​。