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Chemical profiling and antioxidant activity of Equisetum ramosissimum Desf. stem extract, a potential traditional medicinal plant for urinary tract infections
Future Journal of Pharmaceutical Sciences Pub Date : 2021-09-23 , DOI: 10.1186/s43094-021-00339-8
Jeyalatchagan Sureshkumar 1 , Singamoorthy Amalraj 2 , Jayaraj Krupa 2 , Muniappan Ayyanar 2 , Ramar Murugan 3 , Anantharaj Tamilselvan 4 , Venkatraman Sriramavaratharajan 5 , Shailendra S. Gurav 6
Affiliation  

Equisetum ramosissimum Desf. (E. ramosissimum) is a widely used traditional medicinal plant to treat urinary tract infections (UTIs) by ethnic people throughout the world. The utility of the plant in treating urinary-related disorders was evaluated against selected pathogenic bacteria which has major role in causing UTIs. Hence, the present study executed to extract phytochemicals like total phenolics and flavonoids, chemical profiling by GC–MS analysis and to test their antioxidant activity from stem extracts of E. ramosissimum. The extraction process was directed by petroleum ether, chloroform, ethyl acetate, methanol, and aqueous solvents. The GC–MS analysis yielded 24 phytoconstituents with linoleic acid, palmitic acid, nonacosane, hexahydrofarnesyl acetone, and octacosane as major compounds. Methanolic extract yielded maximum amount of phenolics (TPC) and flavonoids (TFC) with 600.02 ± 0.22 mg GAE/g and 631.38 ± 0.69 mg QE/g, respectively. Methanolic extract also exhibited notable free radical scavenging activity with an IC50 of 123.89 ± 0.73, 150.10 ± 1.02, 146.01 ± 0.54, and 63.73 ± 6.12 µg/mL for DPPH, FRAP, ABTS, and O2− assays, respectively. The minimum inhibitory concentration (MIC) required to inhibit the growth of tested pathogenic bacteria was observed in aqueous and methanolic extracts with the value being 31.25 µg/mL against R. equi and V. cholerae. As like, methanolic and petroleum ether extracts efficiently inhibited the growth of B. subtilis with the MIC of 31.25 µg/mL. It was concluded that the notable effect of methanolic and aqueous extracts against the uropathogenic bacteria reported in this study supported the traditional uses of this plant in treating UTIs. The results acquired from this investigation revealed that E. ramosissimum stem extract might be considered as an interesting candidate in the development of antibacterial agent against UTIs coupled with antioxidant properties.

中文翻译:

Equisetum ramosissimum Desf 的化学分析和抗氧化活性。茎提取物,一种潜在的治疗尿路感染的传统药用植物

木贼属 ramosissimum Desf。(E. ramosissimum) 是一种广泛使用的传统药用植物,用于治疗世界各地种族人群的尿路感染 (UTI)。针对在引起 UTI 中起主要作用的选定病原菌评估了该植物在治疗泌尿相关疾病中的效用。因此,本研究旨在提取植物化学物质,如总酚类和黄酮类化合物,通过 GC-MS 分析进行化学分析,并从 E. ramosissimum 的茎提取物中测试它们的抗氧化活性。萃取过程由石油醚、氯仿、乙酸乙酯、甲醇和水性溶剂指导。GC-MS 分析产生了 24 种植物成分,主要化合物为亚油酸、棕榈酸、壬二烷、六氢法呢基丙酮和二十八烷。甲醇提取物产生最大量的酚类物质 (TPC) 和黄酮类化合物 (TFC),分别为 600.02 ± 0.22 mg GAE/g 和 631.38 ± 0.69 mg QE/g。甲醇提取物还表现出显着的自由基清除活性,对 DPPH、FRAP、ABTS 和 O2- 测定的 IC50 分别为 123.89 ± 0.73、150.10 ± 1.02、146.01 ± 0.54 和 63.73 ± 6.12 µg/mL。在水提取物和甲醇提取物中观察到抑制被测病原菌生长所需的最低抑菌浓度 (MIC),对马蹄疫菌和霍乱弧菌的抑制浓度为 31.25 µg/mL。同样,甲醇和石油醚提取物有效抑制枯草芽孢杆菌的生长,MIC 为 31.25 µg/mL。得出的结论是,本研究中报道的甲醇和水提取物对尿路致病菌的显着效果支持了这种植物在治疗 UTI 中的传统用途。从这项调查中获得的结果表明,E. ramosissimum 茎提取物可能被认为是开发抗 UTI 抗菌剂并具有抗氧化特性的有趣候选物。
更新日期:2021-09-24
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