Abstract

In this study, silver nanoparticles (AgNPs) were synthesized economically and simply using an environmentally friendly method with the extract obtained from agricultural waste olive leaves. AgNPs synthesized according to the analysis data were determined to have maximum absorbance at 433.5 nm wavelength, spherical appearance, 7.2 nm crystal nano size and −19.9 mV zeta potential. It was determined by the microdilution method with Minimum Inhibition Concentration (MIC) that AgNPs exert a suppressive effect on the growth of pathogen gram-negative, positive bacteria and yeast at very low concentrations. The cytotoxic effects of the particles were investigated on healthy cell lines (HDF) and cancerous cell lines (U118, CaCo-2, Skov-3). AgNPs showed up to 70% suppression in cancer cell lines.

摘要

在这项研究中，银纳米粒子 (AgNPs) 采用环保的方法，从农业废弃橄榄叶中提取，经济而简单地合成。根据分析数据合成的 AgNPs 在 433.5 nm 波长处具有最大吸光度、球形外观、7.2 nm 晶体纳米尺寸和 -19.9 mV zeta 电位。通过最小抑制浓度 (MIC) 的微量稀释法确定，AgNPs 在非常低的浓度下对病原体革兰氏阴性、阳性细菌和酵母的生长具有抑制作用。研究了颗粒对健康细胞系 (HDF) 和癌细胞系 (U118、CaCo-2、Skov-3) 的细胞毒性作用。AgNPs 在癌细胞系中表现出高达 70% 的抑制。