Metagenomic sequencing, basing on whole genomic analysis, was carried out to explain the taxonomic and functional patterns of microbial communities in co-digestion and mono-digestions with corn straw and pig manure. Bacteroides graminisolvens and Clostridium stercorarium, the dominant species for corn straw degradation, and Proteiniphilum acetatigenes, emphasizing on protein decomposition, performed the optimum abundance during the co-digestion to improve efficiency of substrate decomposition. Methanosaeta concilii was the richest species during the process of bio-methane production, whose quantity and status in co-digestion were better than in mono-digestion. Analysis of functional patterns indicated that the carbohydrate-active enzymes in co-digestion were sustained abundant resulting short lag time, and the abundance of enzymes involving in methanogenesis were extremely high in co-digestion indicating the active methane metabolism. An adequate understanding of taxonomic and functional patterns further revealed the mechanism of rapid startup and superb methane production in co-digestion.

基于全基因组分析的宏基因组测序被用来解释与玉米秸秆和猪粪共同消化和单一消化中微生物群落的分类和功能模式。Bacteroides graminisolvens和Clostridium stercorarium（玉米秸秆降解的主要物种）和Proteiniphilum acetatigenes强调蛋白质分解，在共消化过程中表现出最佳丰度，以提高底物分解效率。产甲烷菌是生物甲烷生产过程中最丰富的物种，其数量和状态在共同消化中优于单一消化。功能模式分析表明，共消化中碳水化合物活性酶持续丰富，导致滞后时间短，并且在共消化中参与产甲烷的酶丰度极高，表明甲烷代谢活跃。对分类和功能模式的充分理解进一步揭示了共同消化中快速启动和产生大量甲烷的机制。