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Impact of stunning before slaughter on expression of skeletal muscles proteome in sheep
Animal Biotechnology ( IF 3.7 ) Pub Date : 2021-09-24 , DOI: 10.1080/10495398.2021.1976198
Mohan Kiran 1 , Naveena B Maheswarappa 2 , Rituparna Banerjee 2 , Venkatesh Ch 3 , Srikanth Rapole 3
Affiliation  

Abstract

The commercial production of halal and kosher meat and controversy surrounding the slaughter without stunning is rapidly growing across the globe. Huge global market for halal and kosher meat warrants conciliation of religious practices and animal welfare for the betterment of meat industry. In the present study, we investigated changes in muscle proteome of sheep (Ovis aries) subjected to either electrical stunning and slaughtering or slaughter without any stunning (halal). The 2DE gel analysis detected approximately 377 protein spots in which 243 (119 up regulated and 124 down regulated) protein spots were significantly (p ≤ 0.05) differentially expressed with a fold change ratio ≥1.5/≤1.5. The in-gel digestion and MALDI-TOF/TOF MS analysis of statistically significant protein spots revealed 35 differentially abundant proteins out of which 26 were up-regulated and 9 were down-regulated. The study demonstrated that slaughtering of sheep without stunning resulted in changes in the abundance of proteins involved in catalytic, structural, and stress related processes. This understanding of protein alterations in sheep slaughtered with and without stunning have the potential to act as possible signature for animal welfare index.



中文翻译:

屠宰前击昏对绵羊骨骼肌蛋白质组表达的影响

摘要

清真和犹太肉类的商业化生产以及围绕未经击晕的屠宰的争议正在全球范围内迅速增长。巨大的全球清真和犹太肉类市场需要协调宗教习俗和动物福利,以改善肉类工业。在本研究中,我们研究了遭受电击晕和屠宰或未经任何击晕(清真)屠宰的绵羊( Ovis aries )肌肉蛋白质组的变化。2DE 凝胶分析检测到大约 377 个蛋白质点,其中 243 个(119 个上调和 124 个下调)蛋白质点显着(p ≤ 0.05) 差异表达倍数变化率≥1.5/≤1.5。对具有统计显着性的蛋白质点进行凝胶内消化和 MALDI-TOF/TOF MS 分析,揭示了 35 个差异丰富的蛋白质,其中 26 个上调,9 个下调。研究表明,未经击晕的羊屠宰会导致参与催化、结构和应激相关过程的蛋白质丰度发生变化。对经击晕和未经击晕屠宰的绵羊蛋白质变化的了解有可能成为动物福利指数的标志。

更新日期:2021-09-24
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