One major difficulty in identifying the gelatinous bodied bullet-shaped Siphonophore, Diphyids, is that their shape is deformed following ethanol fixation. Ethanol often is preferred over other fixatives, since samples fixed in ethanol can be used for molecular studies that can supplement morphological findings. To overcome this problem, we obtained protein mass spectra of ten species of Diphyidae found in the waters of the Kuroshio Current (Northwest Pacific and South Coast of South Korea) to test whether MALDI-ToF MS could be used as a methodology for species identification. In addition, a number of morphological characteristics that can be used with ethanol-treated samples was summarized. Concatenated phylogenetic analysis was also performed to determine the phylogenetic relationship by obtaining partial sequences of four genes (mtCOI, 16S rRNA, 18S rRNA, and ITS regions). Based on our integrative analysis, MALDI-ToF MS was evaluated as a potentially fast, inexpensive, and accurate tool for species identification along with conventional morphological and DNA barcoding for Diphyidae.

识别凝胶体子弹形虹吸管 Diphyids 的一个主要困难是它们的形状在乙醇固定后变形。乙醇通常优于其他固定剂，因为固定在乙醇中的样品可用于分子研究，以补充形态学发现。为了克服这个问题，我们获得了在黑潮海流（西北太平洋和韩国南海岸）的水域中发现的十种双鳍鱼的蛋白质质谱，以测试 MALDI-ToF MS 是否可以用作物种鉴定的方法。此外，还总结了一些可用于乙醇处理样品的形态学特征。还进行了连锁系统发育分析，通过获得四个基因（mtCOI、16S rRNA、18S rRNA 和 ITS 区域）。根据我们的综合分析，MALDI-ToF MS 被评估为一种潜在的快速、廉价和准确的物种鉴定工具，以及双翅目的常规形态学和 DNA 条形码。