Industrial Crops and Products ( IF 5.9 ) Pub Date : 2021-09-24 , DOI: 10.1016/j.indcrop.2021.114074 Lara M. Campos 1 , Ari S.O. Lemos 1 , Priscila L. Paula 1 , Thalita F. Souza 1 , Thiago P. Silva 2 , Vinícius N. Rocha 3 , Ana Carolina M. Apolônio 4 , Priscila F. Pinto 5 , Rossana C.N. Melo 2 , Rodrigo L. Fabri 1
The battery of antifungals available to treat infections caused by Candida albicans is limited and can lead to adverse effects. Treatment of these infections continues to be a challenge mainly because of the Candida resistance to multiple antifungals. The aim of this work was to investigate the antifungal activity of Mitracarpus frigidus (Willd. Ex Roem. Schult.) K. Schum methanolic extract (MFM) against virulence factors of a multidrug-resistant C. albicans strain (ATCC® 10231™). Fungal viability and morphogenesis were evaluated through microscopy techniques associated with molecular markers to detect metabolic activity and pseudohyphae formation. The potential action of MFM against biofilm proliferation and adhesion was investigated with the use of different approaches - whole slide imaging (WSI), which enables quantification of the total biofilm area, scanning electron microscopy (SEM), and analysis of the biochemical composition of the extracellular polymeric matrix. MFM treatment significantly decreased the yeast metabolic activity and the proportion of pseudohyphae. Remarkably, MFM also reduced the C. albicans pre-established biofilm and interfered with its adhesion, as identified by both WSI and SEM. Moreover, MFM affected the biofilm matrix biochemical composition by reducing mainly the concentrations of total carbohydrates and eDNA. Our findings demonstrate that the MFM has a therapeutic potential with good activity against the C. albicans virulence factors by acting against filamentation and biofilm mode of growth of this species.
中文翻译:
Mitracarpus frigidus 的甲醇提取物抑制多药耐药白色念珠菌的成丝和生物膜生长模式
可用于治疗由白色念珠菌引起的感染的一系列抗真菌药是有限的,并可能导致不良反应。这些感染的治疗仍然是一个挑战,主要是因为念珠菌对多种抗真菌药具有抗性。这项工作的目的是研究Mitracarpus frigidus (Willd. Ex Roem. Schult.) K. Schum 甲醇提取物 (MFM) 对多重耐药白色念珠菌毒力因子的抗真菌活性应变(ATCC® 10231™)。真菌活力和形态发生通过与分子标记相关的显微镜技术进行评估,以检测代谢活动和假菌丝形成。使用不同的方法研究了 MFM 对生物膜增殖和粘附的潜在作用 - 全玻片成像 (WSI),它能够量化总生物膜面积、扫描电子显微镜 (SEM) 和分析生物膜的生化成分细胞外聚合物基质。MFM 处理显着降低酵母代谢活性和假菌丝的比例。值得注意的是,MFM 还减少了白色念珠菌预先建立的生物膜并干扰其粘附,如 WSI 和 SEM 所确定的。此外,MFM 主要通过降低总碳水化合物和 eDNA 的浓度来影响生物膜基质的生化成分。我们的研究结果表明,MFM 具有治疗潜力,对白色念珠菌毒力因子具有良好的活性,可通过作用于该物种的丝状形成和生物膜生长模式。