当前位置: X-MOL 学术Nanoscale › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Specific capture and intact release of breast cancer cells using a twin-layer vein-shaped microchip with a self-assembled surface
Nanoscale ( IF 6.7 ) Pub Date : 2021-09-09 , DOI: 10.1039/d1nr04018a
Yixing Gou 1, 2 , Zhuyuan Chen 3 , Changku Sun 1 , Peng Wang 1 , Zheng You 2 , Yaxiaer Yalikun 4, 5 , Yo Tanaka 4 , Dahai Ren 2
Affiliation  

Breast cancer is the most fatal disease among female cancers yet its detection still relies on needle biopsy. The unique physical and immune characteristics of breast cancer cells different from blood cells make them suitable to be employed as excellent biomarkers in liquid biopsy, through which breast cancer cells are collected from peripheral blood for further cancer diagnosis, medical treatment monitoring, and drug screening. Although the separation and enrichment of breast cancer cells from peripheral blood have been studied for years, there are still two problems to be solved in these methods: the low efficiency of on-chip immunologic capture in the flow state and the influence of the conjugated antibodies for the following analyses during cell release. In this paper, a vein-shaped microchip with self-assembled surface was developed for the specific and robust capture (91.2%) of breast cancer cells in the flow state. A protein-recovery process was proposed, in which trypsin served as a mild release reagent, releasing 92% of cells with high viability (96%), normal adherent proliferation, and complete proteins on the cell membrane, avoiding disturbance of the conjugated chemical molecules in the following clinical study. The excellent performance demonstrated in isolating free breast cancer cells from real peripheral blood sample, originating from the orthotopic 4T1 breast cancer metastatic models, suggest the microchip could be utilized as a multiple circulating tumor cell capture and release platform that could allow providing more reliable information in liquid biopsies.

中文翻译:

使用具有自组装表面的双层静脉形微芯片特异性捕获和完整释放乳腺癌细胞

乳腺癌是女性癌症中最致命的疾病,但其检测仍然依赖于针刺活检。乳腺癌细胞不同于血细胞的独特的物理和免疫特性使其适合作为液体活检中优良的生物标志物,通过液体活检从外周血中收集乳腺癌细胞,用于进一步的癌症诊断、医疗监测和药物筛选。虽然从外周血中分离富集乳腺癌细胞已经研究多年,但这些方法仍有两个问题需要解决:流动状态下芯片免疫捕获效率低和偶联抗体的影响用于细胞释放期间的以下分析。在本文中,开发了一种具有自组装表面的静脉形微芯片,用于在流动状态下特异性和稳健地捕获 (91.2%) 的乳腺癌细胞。提出了蛋白质回收过程,其中胰蛋白酶作为温和释放试剂,释放 92% 的细胞具有高活力 (96%)、正常的贴壁增殖和细胞膜上的完整蛋白质,避免共轭化学分子的干扰在接下来的临床研究中。从来自原位 4T1 乳腺癌转移模型的真实外周血样本中分离游离乳腺癌细胞的优异性能表明,该微芯片可用作多循环肿瘤细胞捕获和释放平台,可以提供更可靠的信息。液体活检。2%) 的乳腺癌细胞处于流动状态。提出了一种蛋白质回收过程,其中胰蛋白酶作为温和释放试剂,释放 92% 高活力 (96%)、正常贴壁增殖和细胞膜上完整蛋白质的细胞,避免共轭化学分子的干扰在接下来的临床研究中。从来自原位 4T1 乳腺癌转移模型的真实外周血样本中分离游离乳腺癌细胞的优异性能表明,该微芯片可用作多循环肿瘤细胞捕获和释放平台,可以提供更可靠的信息。液体活检。2%) 的乳腺癌细胞处于流动状态。提出了蛋白质回收过程,其中胰蛋白酶作为温和释放试剂,释放 92% 的细胞具有高活力 (96%)、正常的贴壁增殖和细胞膜上的完整蛋白质,避免共轭化学分子的干扰在接下来的临床研究中。从来自原位 4T1 乳腺癌转移模型的真实外周血样本中分离游离乳腺癌细胞的优异性能表明,该微芯片可用作多循环肿瘤细胞捕获和释放平台,可以提供更可靠的信息。液体活检。释放92%高活力(96%)、正常贴壁增殖和细胞膜上完整蛋白质的细胞,避免后续临床研究中结合化学分子的干扰。从来自原位 4T1 乳腺癌转移模型的真实外周血样本中分离游离乳腺癌细胞的优异性能表明,该微芯片可用作多循环肿瘤细胞捕获和释放平台,可以提供更可靠的信息。液体活检。释放92%高活力(96%)、正常贴壁增殖和细胞膜上完整蛋白质的细胞,避免后续临床研究中结合化学分子的干扰。从来自原位 4T1 乳腺癌转移模型的真实外周血样本中分离游离乳腺癌细胞的优异性能表明,该微芯片可用作多循环肿瘤细胞捕获和释放平台,可以提供更可靠的信息。液体活检。
更新日期:2021-09-24
down
wechat
bug