Hypochlorous acid (HClO) is an important bactericide, and adjusting the content of HClO helps to improve the host's innate immunity and resist microbial invasion. Aggregation-induced luminescence (AIE) is the opposite of aggregation-induced quenching (ACQ). Compounds with AIE properties emit weakly in a dispersed state in solution and they can emit strong fluorescence in an aggregated state. In this article, we proposed a new AIE fluorescent probe QM-ClO based on the quinoline-malononitrile (QM) fluorophore and dimethylthiocarbamate (DMTC) to detect HClO. The probe QM-ClO showed a fast response time, a low detection limit of 30.8 nM and a large Stokes shift (190 nm). Carbonyl cyanide metachlorophenyl-hydrazone (CCCP) was used to induce cell apoptosis, and then an increase in the HClO content was observed in the cell. It is proved that cell apoptosis can lead to the increase of the HClO content in the cell. This probe provides an effective tool for studying apoptosis-related diseases.

中文翻译：

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用于 HClO 和细胞凋亡成像的长波长可激活 AIEgen 荧光探针

次氯酸（HClO）是一种重要的杀菌剂，调节HClO的含量有助于提高宿主的先天免疫力，抵抗微生物入侵。聚集诱导发光 (AIE) 与聚集诱导猝灭 (ACQ) 相反。具有 AIE 特性的化合物在溶液中以分散状态发出微弱的荧光，而在聚集状态下发出强烈的荧光。在本文中，我们提出了一种基于喹啉-丙二腈 (QM) 荧光团和二甲基硫代氨基甲酸酯 (DMTC)的新型 AIE 荧光探针QM-ClO来检测 HClO。探针QM-ClO显示出快速响应时间、30.8 nM 的低检测限和大斯托克斯位移 (190 nm)。使用羰基氰化物间氯苯腙（CCCP）诱导细胞凋亡，然后观察到细胞中HClO含量的增加。证明细胞凋亡可导致细胞内HClO含量增加。该探针为研究细胞凋亡相关疾病提供了有效工具。