A novel clade of RNA viruses was identified in the mammalian gastrointestinal tract by next-generation sequencing. Phylogenetically, these viruses are related to the genera Tombusviridae (plant viruses) and Flaviviridae, which includes mammalian, avian and insect hosts. Named in line with their characterization as stool-associated Tombus-like viruses, it is unclear if statoviruses infect mammals or are dietary in origin. Here, metagenomic sequencing of faecal material collected from a 10-week-old calf with enteric disease found that 20 % of the reads mapped to a de novo-assembled 4 kb contig with homology to statoviruses. Phylogenetic analysis of the statovirus genome found a clear evolutionary relationship with statovirus A, but, with only 47 % similarity, we propose that the statovirus sequence presents a novel species, statovirus F. A TaqMan PCR targeting statovirus F performed on faecal material found a cycle threshold of 11, suggesting a high titre of virus shed from the calf with enteric disease. A collection of 48 samples from bovine enteric disease diagnostic submissions were assayed by PCR to investigate statovirus F prevalence and 6 of 48 (12.5 %) were positive. An ELISA to detect antibodies to the coat protein found that antibodies to statovirus F were almost ubiquitous in bovine serum. Combined, the PCR and ELISA results suggest that statovirus F commonly infects cattle. Further research is needed to elucidate the aetiological significance of statovirus infection.

中文翻译：

---

在患有肠道疾病的小牛粪便样本中鉴定出一种新型状态病毒

通过下一代测序在哺乳动物胃肠道中鉴定出一种新的 RNA 病毒进化枝。在系统发育上，这些病毒与包括哺乳动物、鸟类和昆虫宿主在内的Tombusviridae（植物病毒）和Flaviviridae属有关。命名符合其作为表征ST OOL -a ssociated要MBUS样病毒，目前还不清楚，如果statoviruses感染哺乳动物或者是起源于饮食。在这里，从一头患有肠道疾病的 10 周大的小牛身上收集的粪便材料的宏基因组测序发现，20% 的读数映射到了从头-组装的 4 kb contig 与状态病毒具有同源性。状态病毒基因组的系统发育分析发现与状态病毒 A 存在明显的进化关系，但是，只有 47% 的相似性，我们认为状态病毒序列呈现了一个新物种，状态病毒 F。针对粪便材料进行的针对状态病毒 F 的 TaqMan PCR 发现了一个循环阈值为 11，表明从患有肠道疾病的小牛身上脱落的病毒滴度很高。对来自牛肠道疾病诊断提交的 48 个样本进行了 PCR 分析，以调查状态病毒 F 的流行情况，48 个样本中有 6 个（12.5%）呈阳性。一项检测外壳蛋白抗体的 ELISA 发现，状态病毒 F 的抗体在牛血清中几乎无处不在。结合起来，PCR 和 ELISA 结果表明状态病毒 F 通常感染牛。