Quorum sensing (QS) and biofilm formation inhibition activity of esculetin on Aeromonas hydrophila SHAe 115 were evaluated. Exposure to esculetin at 25, 50, and 100μg/ml significantly inhibited the production of protease and hemolysin, the formation of biofilms and attenuated the swarming motility of A. hydrophila SHAe 115. Biofilm forming inhibition was also observed through confocal laser scanning microscopy and scanning electron microscope. Quantitative real-time PCR analysis indicated that genes positively related to QS and biofilm formation were downregulated to varying degrees, while gene (litR) negatively related to biofilm formation was significantly upregulated. The phenotypic results were in good agreement with gene expression levels. These results indicated that esculetin would be a potential QS inhibitor for A. hydrophila.

七叶树素的群体感应（QS）和生物膜形成抑制活性 嗜水气单胞菌SHAe 115 被评估。暴露于 25、50 和 100μg/ml 的七叶亭可显着抑制蛋白酶和溶血素的产生、生物膜的形成并减弱嗜水 A.SHAe 115。还通过共聚焦激光扫描显微镜和扫描电子显微镜观察到生物膜形成抑制。定量实时 PCR 分析表明，与 QS 和生物膜形成正相关的基因不同程度地下调，而基因（升) 与生物膜形成负相关的显着上调。表型结果与基因表达水平非常一致。这些结果表明，七叶亭是一种潜在的 QS 抑制剂，用于嗜水 A..