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Influence of New Compound Disinfectant From N-Dodecyl-2-(Piridin-1-Ium)Acetamide Chloride on Pathogenic Microorganisms in Poultry Houses
Frontiers in Microbiology ( IF 5.2 ) Pub Date : 2021-09-24 , DOI: 10.3389/fmicb.2021.735859
Nannan Chen 1, 2 , Pingwei Qin 2 , Yu Liu 1 , Ying Yang 3 , Hairuo Wen 3 , Lihua Jia 4 , Jing Li 1 , Zhanbo Zhu 1
Affiliation  

With the development of large-scale and intensive poultry farming, environmental disinfection has become particularly important, and the effectiveness of disinfection depends upon the performance of the disinfectants. Quaternate ammonium salt is a group of positively charged polyatomic ions with both antibacterial and antiviral activities. In order to prepare an ideal disinfectant for poultry farms, we combined a quaternate ammonium salt N-dodecyl-2-(piridin-1-ium)acetamide chloride with two other disinfectants (chlorhexidine acetate and glutaraldehyde), respectively. The antimicrobial activity, mutagenicity, and safety of the compound disinfectants were assessed by the European Standard methods using ATCC strains and clinical isolates. The results showed that both compound disinfectants meet the requirements of microbial reduction, and their effectiveness was not affected by organic matter. Quaternary ammonium disinfectant resistance genes were not detected in the strains tested indicating that bacteria are less likely to develop resistance to these compound disinfectants. Ames test showed that there was no detectable mutagenicity in the strains treated with the compound disinfectants. In vivo experiment showed that both compound disinfectants did not have significant pathological effect in mice. The bactericidal effect of the compound disinfectants was not significantly different among strains of different sources (p>0.05). Clinical tests showed that compound disinfectant had a good bactericidal effect on the air and ground of poultry farms. These results show that quaternary ammonium salts in combination with other compounds can enhance the bactericidal effect and can be used safely in poultry feedlots. This study provides a technical reference for the development of a new quaternate ammonium compound disinfectant with strong disinfection effect and low irritation.



中文翻译:

N-Dodecyl-2-(Piridin-1-Ium)乙酰胺氯化物新型复合消毒剂对禽舍病原微生物的影响

随着家禽规模化、集约化养殖的发展,环境消毒变得尤为重要,消毒效果取决于消毒剂的性能。季铵盐是一组带正电荷的多原子离子,具有抗菌和抗病毒活性。为了制备理想的家禽养殖场消毒剂,我们结合了季铵盐N-dodecyl-2-(piridin-1-ium)乙酰胺氯化物分别与其他两种消毒剂(醋酸氯己定和戊二醛)。复合消毒剂的抗菌活性、致突变性和安全性通过欧洲标准方法使用 ATCC 菌株和临床分离株进行评估。结果表明,两种复合消毒剂均满足微生物减少的要求,且其有效性不受有机物的影响。在测试的菌株中未检测到季铵消毒剂抗性基因,表明细菌不太可能对这些复合消毒剂产生抗性。艾姆斯试验表明,经复合消毒剂处理的菌株未检测到致突变性。体内实验表明,两种复方消毒剂对小鼠均无明显病理作用。复合消毒剂的杀菌效果在不同来源的菌株间无显着差异(>0.05)。临床试验表明,复合消毒剂对家禽养殖场的空气和地面有良好的杀菌作用。这些结果表明,季铵盐与其他化合物结合可以增强杀菌效果,可以在家禽饲养场安全使用。本研究为开发一种消毒效果强、刺激性低的新型季铵盐复合消毒剂提供技术参考。

更新日期:2021-09-24
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