当前位置: X-MOL 学术Arch. Virol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
High molecular diversity and divergent subpopulations of the begomovirus cnidoscolus mosaic leaf deformation virus associated with Cnidoscolus urens.
Archives of Virology ( IF 2.7 ) Pub Date : 2021-09-23 , DOI: 10.1007/s00705-021-05245-1
Adso L S F Mendes 1 , Aline M Melo 1 , Roberto Ramos-Sobrinho 2 , Sarah J C Silva 1 , Camila G Ferro 3, 4 , Mayra M M Ferro 1 , F Murilo Zerbini 3 , Gaus S A Lima 1 , Iraildes P Assunção 1
Affiliation  

Begomoviruses have circular, single-stranded DNA genomes encapsidated into twinned quasi-icosahedral particles and are transmitted by whiteflies of the Bemisia tabaci sibling group. Begomoviruses infect cultivated and non-cultivated plants, causing great losses in economically important crops worldwide. To better understand the genetic diversity of begomoviruses infecting the non-cultivated host Cnidoscolus urens, leaf samples exhibiting virus-like symptoms were collected in different localities in the state of Alagoas, Brazil, during 2015 and 2016. Forty-two complete DNA-A sequences were cloned and sequenced by the Sanger method. Based on nucleotide sequence comparisons, the 42 new isolates were identified as the bipartite begomovirus cnidoscolus mosaic leaf deformation virus (CnMLDV). The CnMLDV isolates were clustered in two phylogenetic groups (clusters I and II) corresponding to their sampling areas, and the high value of Wright's F fixation index observed for the DNA-A sequences suggests population structuring. At least seven independent intraspecies recombination events were predicted among CnMLDV isolates, with recombination breakpoints located in the common region (CR) and in the CP and Rep genes. Also, a high per site nucleotide diversity (π) was observed for CnMLDV isolates, with CP being significantly more variable than Rep. Despite the high genetic variability, strong negative or purifying selection was identified as the main selective force acting upon CP and Rep.

中文翻译:

与 Cnidoscolus urens 相关的 begomovirus cnidoscolus 花叶变形病毒的高分子多样性和不同亚群。

Begomoviruses 的环状单链 DNA 基因组被包裹在孪生的准二十面体颗粒中,并由粉虱同胞群的粉虱传播。Begomovirus 感染栽培和非栽培植物,在全球范围内造成经济上重要作物的巨大损失。为了更好地了解感染非栽培宿主 Cnidoscolus urens 的贝戈莫病毒的遗传多样性,在 2015 年和 2016 年期间,在巴西阿拉戈斯州的不同地点收集了表现出病毒样症状的叶子样本。42 个完整的 DNA-A 序列通过 Sanger 方法进行克隆和测序。基于核苷酸序列比较,42个新分离株被鉴定为二分贝戈莫病毒cnidoscolus花叶变形病毒(CnMLDV)。CnMLDV 分离株聚集在与其采样区域相对应的两个系统发育组(簇 I 和 II)中,并且对 DNA-A 序列观察到的 Wright 固定指数的高值表明种群结构。在 CnMLDV 分离株中预测了至少 7 个独立的种内重组事件,重组断点位于共同区域 (CR) 和 CP 和 Rep 基因中。此外,在 CnMLDV 分离株中观察到较高的每个位点核苷酸多样性 (π),其中 CP 的变异性明显高于 Rep。尽管遗传变异性高,但强阴性或纯化选择被确定为作用于 CP 和 Rep 的主要选择力。s 观察到的 DNA-A 序列的 F 固定指数表明群体结构。在 CnMLDV 分离株中预测了至少 7 个独立的种内重组事件,重组断点位于共同区域 (CR) 和 CP 和 Rep 基因中。此外,在 CnMLDV 分离株中观察到较高的每个位点核苷酸多样性 (π),其中 CP 的变异性明显高于 Rep。尽管遗传变异性高,但强阴性或纯化选择被确定为作用于 CP 和 Rep 的主要选择力。s 观察到的 DNA-A 序列的 F 固定指数表明群体结构。在 CnMLDV 分离株中预测了至少 7 个独立的种内重组事件,重组断点位于共同区域 (CR) 和 CP 和 Rep 基因中。此外,在 CnMLDV 分离株中观察到较高的每个位点核苷酸多样性 (π),其中 CP 的变异性明显高于 Rep。尽管遗传变异性高,但强阴性或纯化选择被确定为作用于 CP 和 Rep 的主要选择力。
更新日期:2021-09-23
down
wechat
bug