Alternative splicing (AS) represents a crucial method in mRNA level to regulate gene expression and contributes to the protein complexity. Abnormal splicing has been reported to play roles in several diseases, including cancers. We developed the OncoSplicing database for visualization of survival-associated and differential alternative splicing in 2019. Here, we provide an updated version of OncoSplicing for an integrative view of clinically relevant alternative splicing based on 122 423 AS events across 33 cancers in the TCGA SpliceSeq project and 238 558 AS events across 32 cancers in the TCGA SplAdder project. The new version of the database contains several useful features, such as annotation of alternative splicing-associated transcripts, survival analysis based on median and optimal cut-offs, differential analysis between TCGA tumour samples and adjacent normal samples or GTEx normal samples, pan-cancer views of alternative splicing, splicing differences and results of Cox’PH regression, identification of clinical indicator-relevant and cancer-specific splicing events, and downloadable splicing data in the SplAdder project. Overall, the substantially updated version of OncoSplicing (www.oncosplicing.com) is a user-friendly and registration-free database for browsing and searching clinically relevant alternative splicing in human cancers.

中文翻译：

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OncoSplicing：33 种人类癌症临床相关选择性剪接的更新数据库

可变剪接 (AS) 代表了 mRNA 水平调节基因表达并有助于蛋白质复杂性的关键方法。据报道，异常剪接在包括癌症在内的多种疾病中发挥作用。我们在 2019 年开发了 OncoSplicing 数据库，用于可视化生存相关和差异选择性剪接。在这里，我们提供了 OncoSplicing 的更新版本，用于基于 TCGA SpliceSeq 项目中 33 种癌症的 122 423 个 AS 事件的临床相关选择性剪接的综合视图以及 TCGA SplAdder 项目中 32 种癌症的 238 558 个 AS 事件。新版本的数据库包含几个有用的功能，例如选择性剪接相关转录本的注释、基于中值和最佳截止值的生存分析、TCGA肿瘤样本与相邻正常样本或GTEx正常样本之间的差异分析，选择性剪接的泛癌视图，Cox'PH回归的剪接差异和结果，临床指标相关和癌症特异性剪接事件的识别，以及可下载的剪接数据在 SplAdder 项目中。总体而言，OncoSplicing (www.oncosplicing.com) 的大幅更新版本是一个用户友好且免注册的数据库，用于浏览和搜索人类癌症临床相关的选择性剪接。