This in vitro study sought to assess the effect of repetitive PBMT on the viability of fibroblasts following aPDT with indocyanine green (ICG). In this in vitro experimental study, human gingival fibroblasts (HGFs) were obtained and incubated in a culture medium. After reaching 10 000 cells cm⁻², the cells were divided into five groups of control, aPDT with ICG and 808 nm (energy density of 24 J cm⁻²), PBMT immediately after aPDT, PBMT with 660 nm (energy density of 7.2 J cm⁻²) immediately and 24 h after aPDT and PBMT immediately and 24 and 48 h after aPDT in 48-well plates. Cell viability was evaluated using the methyl thiazolyl tetrazolium (MTT) assay after 1, 4 and 7 days of incubation. Statistical analyses were performed using one-way ANOVA. Cell viability significantly decreased in group 2 (P < 0.002). We observed no significant increase in cell viability at any time point in group 3 (P > 0.05). Cell viability significantly increased in groups 4 and 5 after the first day of incubation (P < 0.000). Emission of 660 nm as PBMT for two and three times along with passage of time would increase the viability of HGFs following aPDT with ICG.

中文翻译：

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吲哚菁绿光动力疗法和 660 nm 光生物调节疗法对成纤维细胞活力的体外影响

这项体外研究旨在评估重复 PBMT 对使用吲哚菁绿 (ICG) 进行 aPDT 后成纤维细胞活力的影响。在这项体外实验研究中，获得了人牙龈成纤维细胞 (HGF) 并在培养基中孵育。达到 10 000 个细胞 cm ^-2后，将细胞分为五组对照，aPDT 与 ICG 和 808 nm（能量密度为 24 J cm ^-2），aPDT 后立即进行 PBMT，PBMT 与 660 nm（能量密度为 7.2 J cm ^-2) 立即和 aPDT 后 24 小时，PBMT 立即和 48 孔板中 aPDT 后 24 和 48 小时。在孵育 1、4 和 7 天后，使用甲基噻唑基四唑 (MTT) 测定法评估细胞活力。使用单因素方差分析进行统计分析。第 2 组的细胞活力显着降低（P  < 0.002）。我们观察到第 3 组任何时间点的细胞活力都没有显着增加（P  > 0.05）。孵育第一天后，第 4 组和第 5 组的细胞活力显着增加（P  < 0.000）。随着时间的推移，660 nm 作为 PBMT 发射 2 次和 3 次会增加 HGF 在 aPDT 与 ICG 后的活力。