Recalcitrant plants are difficult to handle in tissue culture, and this limits their accessibility for various studies. Novel strategies are being developed to compensate the restricted nature of these plants to make them more adapted to tissue culture applications. Sorghum is one of the most recalcitrant plants with decreased efficiency and non-standardized tissue culture protocols. The advances in embryogenic genes ease the somatic embryo formation and regeneration ability in sorghum. BABY BOOM (BBM) is the most prominent transcription factor involved in somatic embryogenesis, even without exogenous auxin application, the spontaneous somatic embryo formation could occur in plants, which makes this gene(s) an optimal target for recalcitrant plants. This study is designed to characterize SbBBM-like genes in sorghum. Two similar length of protein pairs, SbBBM-like 1 (XP_021313568.1) and SbBBM-like 2 (XP_002452443.1), were identified in cv. Aldarı and cis-regulatory elements of these proteins were observed to be involved in particularly light response and hormonal regulation. The protein –protein interaction data indicated possible role in auxin mechanism. The expression data showed SbBBM-like was highly expressed in seedling root and embryogenic callus, however the expression was highest in embryogenic callus for SbBBM-like 2 with almost 200-fold increase, compared to other tissues.

顽固的植物在组织培养中难以处理，这限制了它们对各种研究的可及性。正在开发新的策略来补偿这些植物的局限性，使它们更适合组织培养应用。高粱是最顽固的植物之一，效率降低，组织培养方案不规范。胚性基因的研究提高了高粱体细胞胚的形成和再生能力。BABY BOOM (BBM) 是参与体细胞胚胎发生的最突出的转录因子，即使没有外源生长素的应用，植物中也可能发生自发的体细胞胚胎形成，这使得该基因成为顽固植物的最佳靶点。本研究旨在表征高粱中的 SbBBM 样基因。在 cv. 中鉴定了两个相似长度的蛋白质对，SbBBM-like 1 (XP_021313568.1) 和 SbBBM-like 2 (XP_002452443.1)。观察到这些蛋白质的 Aldarı 和顺式调节元件特别参与光反应和激素调节。蛋白质-蛋白质相互作用数据表明可能在生长素机制中起作用。表达数据显示 SbBBM-like 在幼苗根和胚性愈伤组织中高表达，但 SbBBM-like 2 在胚性愈伤组织中表达最高，与其他组织相比增加了近 200 倍。蛋白质-蛋白质相互作用数据表明可能在生长素机制中起作用。表达数据显示 SbBBM-like 在幼苗根和胚性愈伤组织中高表达，但 SbBBM-like 2 在胚性愈伤组织中表达最高，与其他组织相比增加了近 200 倍。蛋白质-蛋白质相互作用数据表明可能在生长素机制中起作用。表达数据显示 SbBBM-like 在幼苗根和胚性愈伤组织中高表达，但 SbBBM-like 2 在胚性愈伤组织中表达最高，与其他组织相比增加了近 200 倍。