Protein-based nanoparticles are promising candidates to be used as nanomedicine due to their high biocompatibility. On the other hand, functional nanoagents are fabricated using complex, nonscalable, and environmentally unfriendly procedures. An urge to meet multiple biomedical and industrial requirements highlights a need to find a universal, eco-friendly, and flexible synthetic strategy of functional nanoagent engineering. Here, we show a green, ultrafacile, and size-controlled synthesis (swift thermal formation; STF) of monodisperse immunoglobulin-based nanoparticles. The synthesis allows the fabrication of hybrid nanoparticles as well. We show that the nanoparticles retain the specificity of immunoglobulins and can target malignant cells. In addition, we demonstrate that the nanoparticles are biocompatible and applicable in vivo. Tunability, ease, and sustainability of the synthesis may turn the targeted nanoagent design into a routine procedure. This ready-to-use solution could assist a broad spectrum of biomedical applications, e.g., imaging and therapy.

中文翻译：

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通过快速热形成在体内合成尺寸控制的基于生物相容性免疫球蛋白的纳米颗粒

基于蛋白质的纳米粒子由于其高生物相容性而成为有希望用作纳米药物的候选者。另一方面，功能性纳米试剂是使用复杂、不可扩展且对环境不友好的程序制造的。满足多种生物医学和工业要求的迫切需求凸显了需要找到一种通用、环保且灵活的功能性纳米试剂工程合成策略。在这里，我们展示了基于单分散免疫球蛋白的纳米粒子的绿色、超简便和尺寸控制的合成（快速热形成；STF）。该合成也允许制造混合纳米粒子。我们表明纳米颗粒保留了免疫球蛋白的特异性并且可以靶向恶性细胞。此外，我们证明纳米颗粒具有生物相容性和适用性体内。合成的可调谐性、简易性和可持续性可能会将目标纳米试剂设计变成常规程序。这种即用型解决方案可以帮助广泛的生物医学应用，例如成像和治疗。