Graphene oxide (GO) is well known for its photo-reactivity with potential reduction under ultraviolet (UV) radiation. GO induces cytotoxicity in cancer cells even though the mechanism involved has not been firmly established when exposed to cancer cells. Therefore, to investigate the potential interaction of GO with UV and their impact on cancer cells, MCF-7 human breast cancer cells were exposed to GO, UV, or in combination, and the cellular impacts associated with these exposures have been identified. UV-irradiated GO showed differences in appearance, UV-absorbance, FTIR, and Raman spectra, indicating substantial changes in UV-irradiated GO structure compared to the non-irradiated GO. Furthermore, UV-irradiated GO reduced the cell viability of MCF-7 cells and increased the cellular level of ROS in the cancer cells that were dependent on the UV exposure time and GO concentration, respectively. Moreover, a synergistic increase in cellular ROS was observed when MCF-7 cells were treated with different GO concentrations followed by UV-irradiation. The synergistic interaction of GO and UV induced apoptotic cell death in MCF-7 cells as evidenced by the increases in MCF-7 cells exhibiting round cell morphology, nuclei condensation, activation of BAX and Caspase as well as the release of Cyt. C from mitochondria, suggesting the potential of UV-irradiated GO in cancer treatment.

氧化石墨烯 (GO) 以其在紫外线 (UV) 辐射下具有潜在还原性的光反应性而闻名。GO 在癌细胞中诱导细胞毒性，尽管在暴露于癌细胞时所涉及的机制尚未确定。因此，为了研究 GO 与紫外线的潜在相互作用及其对癌细胞的影响，MCF-7 人类乳腺癌细胞暴露于 GO、紫外线或组合，并且已经确定了与这些暴露相关的细胞影响。经紫外线照射的 GO 在外观、紫外吸光度、FTIR 和拉曼光谱方面表现出差异，表明与未照射的 GO 相比，经紫外线照射的 GO 结构发生了实质性变化。此外，紫外线照射的 GO 降低了 MCF-7 细胞的细胞活力并增加了癌细胞中 ROS 的细胞水平，这分别取决于紫外线照射时间和 GO 浓度。此外，当 MCF-7 细胞用不同的 GO 浓度处理，然后进行紫外线照射时，观察到细胞 ROS 的协同增加。GO 和 UV 的协同相互作用在 MCF-7 细胞中诱导凋亡细胞死亡，这可以通过表现出圆形细胞形态、细胞核浓缩、BAX 和 Caspase 激活以及 Cyt 释放的 MCF-7 细胞的增加来证明。来自线粒体的 C，表明紫外线照射的 GO 在癌症治疗中的潜力。当 MCF-7 细胞用不同的 GO 浓度处理，然后用紫外线照射时，观察到细胞 ROS 的协同增加。GO 和 UV 的协同相互作用在 MCF-7 细胞中诱导凋亡细胞死亡，这可以通过表现出圆形细胞形态、细胞核浓缩、BAX 和 Caspase 激活以及 Cyt 释放的 MCF-7 细胞的增加来证明。来自线粒体的 C，表明紫外线照射的 GO 在癌症治疗中的潜力。当 MCF-7 细胞用不同的 GO 浓度处理，然后用紫外线照射时，观察到细胞 ROS 的协同增加。GO 和 UV 的协同相互作用在 MCF-7 细胞中诱导凋亡细胞死亡，这可以通过表现出圆形细胞形态、细胞核浓缩、BAX 和 Caspase 激活以及 Cyt 释放的 MCF-7 细胞的增加来证明。来自线粒体的 C，表明紫外线照射的 GO 在癌症治疗中的潜力。