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Determination of Raspberry Cultivar Authenticity Based on Multiplexed Microsatellite Fingerprinting
International Journal of Fruit Science ( IF 2.4 ) Pub Date : 2021-09-22 , DOI: 10.1080/15538362.2021.1975011
Metka Šiško 1 , Anton Ivančič 1 , Andrej Šušek 1
Affiliation  

ABSTRACT

Raspberries (Rubus idaeus L.) are important vegetatively propagated fruit plants. They are used as food, as medicinal plants, and in pharmaceutical and cosmetic industry. For the protection of intellectual property rights, it is very important to have efficient methods that enable fast and accurate identification of cultivars. Raspberry cultivars can be differentiated in two ways: on the phenotypic level with morphological descriptors and on the molecular level with molecular markers. Among the frequently used are SSRs, which are highly informative, easy to score and have a good genome coverage. Considering 19 previously published SSR molecular markers, we selected the most specific loci for each selected genotype and developed specific fingerprint for each of the genotypes included in the study: four red raspberry cultivars (‘Polka,’ ‘Glen Ample,’ ‘Meeker,’ ‘Rose de Côte d’Or’) and two genotypes named Sicoly and Dieffenbach. The aim of our investigation was to demonstrate the differentiation between studied raspberry genotypes by specific amplified fragment patterns, which were unique for each genotype and were based on simple sequence repeats (SSRs). Furthermore, the developed fingerprints were successfully tested on randomly chosen, unknown genotypes.



中文翻译:

基于多重微卫星指纹的覆盆子品种真伪鉴定

摘要

覆盆子 ( Rubus idaeusL.) 是重要的无性繁殖果树。它们被用作食品、药用植物以及制药和化妆品工业。为了保护知识产权,拥有能够快速准确识别品种的有效方法非常重要。覆盆子栽培品种可以通过两种方式进行区分:在表型水平上使用形态描述符和在分子水平上使用分子标记。其中经常使用的是 SSR,它们信息量大、易于评分且具有良好的基因组覆盖率。考虑到之前发表的 19 个 SSR 分子标记,我们为每个选定的基因型选择了最具体的基因座,并为研究中包含的每个基因型开发了特定的指纹:四个红树莓栽培品种('Polka'、'Glen Ample'、'Meeker、''Rose de Côte d'Or') 和两种名为 Sicoly 和 Dieffenbach 的基因型。我们调查的目的是通过特定的扩增片段模式证明所研究的覆盆子基因型之间的差异,这些片段模式对于每个基因型都是独一无二的,并且基于简单序列重复 (SSR)。此外,开发的指纹成功地测试了随机选择的未知基因型。

更新日期:2021-09-23
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