Fourier ptychographic microscopy (FPM), as an emerging computational imaging method, has been applied to quantitative phase imaging with resolution bypassing the physical limit of the detection objective. Due to the weak illumination intensity and long image acquisition time, the achieved imaging speed in current FPM methods is still low, making them unsuitable for real-time imaging applications. We propose and demonstrate a high-speed FPM method based on using laser illumination and digital micro-mirror devices for illumination angle scanning. In this new, to the best of our knowledge, FPM method, we realized quantitative phase imaging and intensity imaging at over 42 frames per second (fps) with around 1 µm lateral resolution. The quantitative phase images have revealed membrane height fluctuations of red blood cells with nanometer-scale sensitivity, while the intensity images have resolved subcellular features in stained cancer tissue slices.

中文翻译：

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用于定量相位成像的高速傅立叶 ptychographic 显微镜

傅立叶 ptychographic 显微镜 (FPM) 作为一种新兴的计算成像方法，已应用于定量相位成像，其分辨率绕过了检测目标的物理限制。由于光照强度弱和图像采集时间长，目前的 FPM 方法实现的成像速度仍然较低，不适合实时成像应用。我们提出并演示了一种基于使用激光照明和数字微镜设备进行照明角度扫描的高速 FPM 方法。据我们所知，在这种新的 FPM 方法中，我们实现了每秒 42 帧 (fps) 以上的定量相位成像和强度成像，横向分辨率约为 1 µm。