CMV infection is a significant complication after solid organ transplantation. We used single cell TCR αβ sequencing to determine how memory inflation impacts clonality and diversity of the CMV-responsive CD8 and CD4 T cell repertoire in the first year after transplantation in human subjects. We observed CD8 T cell inflation but no changes in clonal diversity, indicating homeostatic stability in clones. In contrast, the CD4 repertoire was diverse and stable over time, with no evidence of CMV-responsive CD4 T cell expansion. We identified shared CDR3 TCR motifs among patients but no public CMV-specific TCRs. Temporal changes in clonality in response to transplantation and in the absence of detectable viral reactivation suggest changes in the repertoire immediately after transplantation followed by an expansion with stable clonal competition that may mediate protection.

CMV感染是实体器官移植后的重要并发症。我们使用单细胞 TCR αβ 测序来确定在人类受试者移植后的第一年，记忆膨胀如何影响 CMV 反应性 CD8 和 CD4 T 细胞库的克隆性和多样性。我们观察到 CD8 T 细胞膨胀，但克隆多样性没有变化，表明克隆的稳态稳定性。相比之下，随着时间的推移，CD4 库是多样化和稳定的，没有证据表明 CMV 反应性 CD4 T 细胞扩增。我们确定了患者之间共享的 CDR3 TCR 基序，但没有公开的 CMV 特异性 TCR。