Purpose

The purpose of this paper is to study the immobilization of porcine pancreatic lipase (PPL), in an organic matrix by a covalent cross-linking method to sense propylparaben (PP) present in aqueous solution.

Design/methodology/approach

PPL immobilization was performed by the covalent cross-linking method, using bovine serum albumin (BSA) in the presence of saturated glutaraldehyde vapor (GA). The preparation of the enzymatic membrane involves the incorporation of porcine pancreatic lipase (PPL), bovine serum albumin (BSA) and glycerol into a phosphate buffer solution (PBS). Characterization of this sensor was performed by impedance spectroscopy (EIS) and scanning electron microscope (SEM). The effect of experimental conditions such as PPL activity, potential, scan rate, PP concentration, pH and presence of interfering elements were studied by cyclic voltammetry.

Findings

Under the optimal experimental conditions, a number of significant factors were optimized. The method exhibited good linearity in the range of 10^–14 to 10^–9 mol/L with a good correlation coefficient of 0.957, detection limit (LOD) of 3.66 × 10^–15 mol/L and high sensitivity of 1.086 mA mol⁻¹L. The authors also obtained a very good coverage rate of the surface equal to 91.44%, and hydrolytic activity of lipase is evaluated to 26.64 mmol min⁻¹. The stability and the interference were also evaluated. The equivalent circuit used to explain the electrochemical behavior of modified electrode is a Randle circuit.

Practical implications

The main application of biosensors is the detection of biomolecules that are either indicators of a disease. For example, electrochemical biosensing techniques can be used as clinical tools to detect breast tumors, because these compounds (PP) were found in breast tumors.

Originality/value

The result registered in this paper indicates that the developed sensor is an efficient, fast, simple and inexpensive analytical tool that can be used for the analysis of water containing PP.

中文翻译：

---

用于检测对羟基苯甲酸丙酯的基于猪胰腺脂肪酶的酶促生物传感器的开发和表征

目的

本文的目的是研究通过共价交联方法将猪胰脂肪酶 (PPL) 固定在有机基质中，以检测水溶液中存在的对羟基苯甲酸丙酯 (PP)。

设计/方法/方法

PPL 固定是通过共价交联方法进行的，在饱和戊二醛蒸汽 (GA) 存在下使用牛血清白蛋白 (BSA)。酶促膜的制备包括将猪胰脂肪酶 (PPL)、牛血清白蛋白 (BSA) 和甘油掺入磷酸盐缓冲溶液 (PBS) 中。该传感器的表征通过阻抗谱 (EIS) 和扫描电子显微镜 (SEM) 进行。通过循环伏安法研究了 PPL 活性、电位、扫描速率、PP 浓度、pH 值和干扰元素的存在等实验条件的影响。

发现

在最佳实验条件下，对多项重要因素进行了优化。该方法在 10 ^–14至 10 ^–9  mol/L范围内具有良好的线性，相关系数为 0.957，检出限 (LOD) 为 3.66 × 10 ^–15  mol/L，灵敏度为 1.086 mA mol ^-1 L. 作者还获得了非常好的表面覆盖率，达到了 91.44%，脂肪酶的水解活性评估为 26.64 mmol min ^-1。还评估了稳定性和干扰。用于解释修饰电极电化学行为的等效电路是 Randle 电路。

实际影响

生物传感器的主要应用是检测作为疾病指标的生物分子。例如，电化学生物传感技术可用作检测乳腺肿瘤的临床工具，因为这些化合物 (PP) 是在乳腺肿瘤中发现的。

原创性/价值

本文的结果表明，所开发的传感器是一种高效、快速、简单且廉价的分析工具，可用于分析含 PP 的水。