As a new kind of porous organic material, covalent organic framework materials (COFs) have many advantages and have been widely used in catalysis, sensing, drug delivery, gas separation and other fields. In this paper, A COF was successfully prepared based on Schiff base reaction of 1, 3, 6, 8-tetrakis(4-formyl phenyl) pyrene (TFPPy) and 1, 3, 6, 8-tetrakis(4-amino phenyl) pyrene (PyTTA) named as TF-Py COF, and its structure was characterized in detail. By physically combining a pre-prepared three-dimensional carbon material containing nitrogen (3D NCNF-T) and TF-Py COF, followed by deposition of AuNPs, relatively poor conductivity of TF-Py COF was efficiently overcome and a novel electrochemical sensing platform with high sensitivity and high selectivity for simultaneous detection of acetaminophen (ACOP) and 4-aminophenol (4-AP) was successfully constructed. The synergistic effect between TF-Py COF, 3D NCNF-T and AuNPs effectively improves the analytical sensitivity of target analytes. Under optimized experimental conditions, the electrochemical sensing platform has a sensitive volt-ampere response and a wide linear range to both ACOP and 4-AP, and the detection limits are 0.13 μM and 0.05 μM (S/N = 3), respectively. The sensor has been successfully applied to the determination of ACOP and 4-AP in acetaminophen tablets and Yellow River water samples, with high recovery rate, good selectivity and reproducibility, and strong anti-interference ability. It is expected to be widely used in the actual sample detection.

共价有机骨架材料（COFs）作为一种新型多孔有机材料，具有诸多优势，已广泛应用于催化、传感、药物输送、气体分离等领域。本文基于1, 3, 6, 8-四(4-甲酰基苯基)芘(TFPPy)和1, 3, 6, 8-四(4-氨基苯基)的Schiff碱反应成功制备了COF芘（PyTTA）命名为TF-Py COF，对其结构进行了详细表征。通过物理结合预先制备的含氮三维碳材料 (3D NCNF-T) 和 TF-Py COF，然后沉积 AuNP，有效克服了 TF-Py COF 电导率相对较差的问题，成功构建了一种具有高灵敏度和高选择性的新型电化学传感平台，可同时检测对乙酰氨基酚 (ACOP) 和 4-氨基苯酚 (4-AP)。TF-Py COF、3D NCNF-T和AuNPs之间的协同作用有效提高了目标分析物的分析灵敏度。在优化的实验条件下，电化学传感平台对ACOP和4-AP均具有灵敏的伏安响应和较宽的线性范围，检测限分别为0.13 μM和0.05 μM（S/N=3）。该传感器已成功应用于对乙酰氨基酚片和黄河水样中ACOP和4-AP的测定，回收率高，选择性和重现性好，并且抗干扰能力强。有望在实际样品检测中得到广泛应用。