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Responsiveness of fecal immunoglobulin A to HPA-axis activation limits its use for mucosal immunity assessment
American Journal of Primatology ( IF 2.4 ) Pub Date : 2021-09-23 , DOI: 10.1002/ajp.23329
Verena Behringer 1 , Nadine Müller-Klein 2, 3 , Christina Strube 4 , Oliver Schülke 3, 5, 6 , Michael Heistermann 1 , Julia Ostner 3, 5, 6
Affiliation  

The assessment of mucosal immunity as a component of animal health is an important aspect for the understanding of variation in host immunity, and its tradeoff against other life-history traits. We investigated immunoglobulin A (IgA), the major type of antibody associated with mucosal immunity, in relation to changes in parasitic burden following anthelminthic treatment in noninvasively collected fecal samples in a semi-free ranging group of Barbary macaques (Macaca sylvanus). We measured IgA in 340 fecal samples of fourteen females and nine males. As IgA has been found to be responsive to stressors, we also related fecal IgA (fIgA) levels to fecal glucocorticoid metabolites (fGCM) measured in the same samples as part of a previous study. We found a high variability within and between individual fIgA levels over time. Running generalized additive mixed models, we found that fIgA levels were higher in males than in females, but did not change in response to the anthelmintic treatment and the resulting reduction in worm burden. Instead, fIgA level changes were significantly correlated to changes in fGCM levels. Our findings indicate that due to the strong responsiveness of fIgA to HPA-axis activity, the measurement of fIgA may have certain limitations with respect to reflecting gastrointestinal parasitic burden. Moreover, the responsiveness of fIgA to stressors interferes with the interpretation of IgA levels in fecal samples as a measure of mucosal immunity, at least in our study population of the Barbary macaques.

中文翻译:

粪便免疫球蛋白 A 对 HPA 轴激活的反应限制了其在粘膜免疫评估中的应用

评估粘膜免疫作为动物健康的一个组成部分是了解宿主免疫变异及其与其他生活史特征的权衡的一个重要方面。我们研究了与黏膜免疫相关的主要抗体类型免疫球蛋白 A (IgA) 与巴巴里猕猴 ( Macaca sylvanus)。我们在 14 名女性和 9 名男性的 340 个粪便样本中测量了 IgA。由于已发现 IgA 对压力源有反应,我们还将粪便 IgA (fIgA) 水平与在先前研究的相同样本中测量的粪便糖皮质激素代谢物 (fGCM) 相关联。我们发现随着时间的推移,个体 fIgA 水平内部和之间存在很大差异。运行广义加性混合模型,我们发现男性的 fIgA 水平高于女性,但并未因驱虫剂治疗和由此产生的蠕虫负担减少而改变。相反,fIgA 水平的变化与 fGCM 水平的变化显着相关。我们的研究结果表明,由于 fIgA 对 HPA 轴活性的强烈反应,fIgA 的测量在反映胃肠道寄生虫负担方面可能存在一定的局限性。
更新日期:2021-11-29
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