Candida albicans is the most common human fungal pathogen that has developed extensive virulence factors which allows successful colonization and infection of the host. Anti-virulence agents can alleviate the pathogenesis of fungi and help the immune system to eradicate them easily. This study aimed to explore the anti-virulence effect of domperidone and candesartan against C. albicans standard strain. Sub-inhibitory concentrations (1/4 and 1/8 of minimum inhibitory concentration) of domperidone and candesartan significantly inhibited the virulence factors hemolysin, lipase, protease, phospholipase, and bioflim formation. It was found that candesartan inhibited biofilm formation by 60.48–67.91%, hemolysin activity (61.21–74.14%), phospholipase activity (40–49.67%), lipase activity (58.97–73%), and protease activity (52.63%), while domperidone was found to inhibit biofilm formation by 70.54–77.49%, hemolysin activity (64.84–69.84%), phospholipase activity (49.67–60%), lipase activity (50–54.87%), and protease activity (52.63–57.9%). Quantitative real time-PCR confirmed the anti-virulence activity of domperidone and candesartan as both drugs significantly reduce the expression of the virulence genes SAP2, SAP6, PLB1, PLB2, LIP4, LIP5. In conclusion, domperidone and candesartan could serve as anti-virulence agents for treatment of C. albicans infections.

白色 念珠菌是最常见的人类真菌病原体，它具有广泛的毒力因子，可以成功地定植和感染宿主。抗毒剂可以减轻真菌的发病机制，帮助免疫系统轻松消灭它们。本研究旨在探索多潘立酮和坎地沙坦对反毒性作用C. 白色念珠菌标准应变。多潘立酮和坎地沙坦的亚抑制浓度（最小抑制浓度的 1/4 和 1/8）显着抑制毒力因子溶血素、脂肪酶、蛋白酶、磷脂酶和生物膜形成。发现坎地沙坦抑制生物膜形成 60.48-67.91%、溶血素活性 (61.21-74.14%)、磷脂酶活性 (40-49.67%)、脂肪酶活性 (58.97-73%) 和蛋白酶活性 (52.63%)，而发现多潘立酮抑制生物膜形成 70.54–77.49%、溶血素活性 (64.84–69.84%)、磷脂酶活性 (49.67–60%)、脂肪酶活性 (50–54.87%) 和蛋白酶活性 (52.63–57.9%)。定量实时 PCR 证实了多潘立酮和坎地沙坦的抗毒力活性，因为这两种药物均显着降低毒力基因SAP2的表达、SAP6、PLB1、PLB2、LIP4、LIP5。总之，多潘立酮和坎地沙坦可作为治疗抗毒性药物C. 白色念珠菌感染。