Adsorption of calf serum organic matter from a phosphate-buffered solution was studied using the electrochemical quartz crystal microbalance with additional dissipation measurements. Two types of crystal surfaces were used: one rough with micrometer-range surface features and one with roughness in the low nanometer range. The results showed that the adsorption of the organic material was about 1.5 orders of magnitude larger on the rough surface and almost independent of serum concentration in the electrolyte. The adsorption rates were found to increase with increasing serum concentration. For rough crystals, the adsorption kinetics were interpreted with the Johnson–Mehl–Avrami–Kolmogorov model, indicating an initial growth phase according to the tⁿ-law, followed by a slower growth as the nucleation sites fill up. This study suggests that specific surface sites are critical to promote adsorption of proteins on a titanium surface.

中文翻译：

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电化学石英晶体微天平耗散技术研究纳米和微米级粗糙度钛表面有机物的吸附

使用带有额外耗散测量的电化学石英晶体微量天平研究了磷酸盐缓冲溶液中小牛血清有机物的吸附。使用了两种类型的晶体表面：一种具有微米范围表面特征的粗糙表面，另一种具有低纳米范围的粗糙度。结果表明，有机材料在粗糙表面上的吸附量大约增加了 1.5 个数量级，并且几乎与电解质中的血清浓度无关。发现吸附率随着血清浓度的增加而增加。对于粗糙晶体，吸附动力学用 Johnson-Mehl-Avrami-Kolmogorov 模型解释，表明根据t ^{n的初始生长阶段}-law，随后随着成核位点的填满而缓慢增长。这项研究表明，特定的表面位点对于促进蛋白质在钛表面的吸附至关重要。