Electrospinning represents the simplest approach to fabricate nanofiber scaffolds that approximate the heterogeneous fibrous structure of the meniscus. More effort is needed to understand the relationship between scaffold properties and cell responses to determine the appropriate scaffolds supporting meniscus tissue repair and regeneration. In this study, we investigate the influence of nanofiber configuration of electrospun scaffolds on phenotype and matrix production of meniscus cells, as well as on scaffold degradation behaviors and biocompatibility. Twisting electrospun nanofibers into yarns not only recapitulates the major collagen bundles of the meniscus but also increases the pore size and porosity of resultant scaffolds. The yarn scaffold significantly regulated expression levels of meniscus-associated genes and promoted extracellular matrix production compared with conventional electrospun scaffolds with random or aligned nanofiber orientation. Additionally, the yarn scaffold allowed considerable cell infiltration and experienced faster degradation and tissue remodeling upon subcutaneous implantation in a rat model. These results suggest that nanofiber configuration dictates cell interactions, scaffold degradation and integration with host tissue, providing design parameters of porosity and pore size of electrospun scaffolds toward meniscus repair.

静电纺丝是制造近似半月板异质纤维结构的纳米纤维支架的最简单方法。需要更多的努力来了解支架特性和细胞反应之间的关系，以确定支持半月板组织修复和再生的适当支架。在这项研究中，我们研究了静电纺丝支架的纳米纤维配置对半月板细胞的表型和基质产生以及支架降解行为和生物相容性的影响。将电纺纳米纤维捻成纱线不仅概括了半月板的主要胶原束，而且还增加了所得支架的孔径和孔隙率。与具有随机或对齐纳米纤维取向的常规电纺支架相比，纱线支架显着调节半月板相关基因的表达水平并促进细胞外基质的产生。此外，纱线支架允许大量细胞浸润，并在大鼠模型中皮下植入后经历更快的降解和组织重塑。这些结果表明，纳米纤维配置决定了细胞相互作用、支架降解和与宿主组织的整合，为半月板修复提供了电纺支架的孔隙率和孔径设计参数。纱线支架允许大量细胞浸润，并在大鼠模型中皮下植入后经历更快的降解和组织重塑。这些结果表明，纳米纤维配置决定了细胞相互作用、支架降解和与宿主组织的整合，为半月板修复提供了电纺支架的孔隙率和孔径设计参数。纱线支架允许大量细胞浸润，并在大鼠模型中皮下植入后经历更快的降解和组织重塑。这些结果表明，纳米纤维配置决定了细胞相互作用、支架降解和与宿主组织的整合，为半月板修复提供了电纺支架的孔隙率和孔径设计参数。