ABSTRACT

In order to explore the effect of the combination of microorganisms and castor (Ricinus communis L.) in remediating copper-contaminated soil, a pot experiment was conducted to study the synergistic effect of Acinetobacter calcoaceticus, as the rhizospheric microorganisms added to siol, on the copper uptaking by castor. The results showed that after 120 days of thinning, the shoot and root biomass of castor added with A. calcoaceticus increased increased by 22.55% and 17.82%, respectively, and the copper content increased by 18.47% and 27.88%, compared with the control (without microorganism addition). The addition of the rhizobacteria could promote the synthesis of chlorophyll, especially chlorophyll a. The SOD and POD were significantly activated at the treatment group, however, which showed a downward trend with the extension of time. Furthermore, A. calcoaceticus could stimulate the secretion of malic acid, oxalic acid, and tartaric acid obviously of the plant, and accelerated the activity of urease, catalase, acid phosphatase and sucrase in the soil. Obove result indicated that the addition of microorganisms not only promoted the growth of castor seedling and increased the tolerance of castor to copper, but also strengthened the accumulation of copper in castor.

摘要

为探讨微生物与蓖麻联合修复铜污染土壤的效果，通过盆栽试验研究了作为根际微生物添加到土壤中的醋酸钙不动杆菌对铜污染土壤的协同作用。蓖麻吸收铜。结果表明，间苗120 d后，添加醋酸钙的蓖麻地上部和根部生物量与对照（不添加微生物）相比，分别增加了22.55%和17.82%，铜含量分别增加了18.47%和27.88%。根际菌的加入可以促进叶绿素的合成，尤其是叶绿素a。治疗组SOD和POD明显激活，但随时间延长呈下降趋势。此外，A. calcoaccius能明显刺激植物分泌苹果酸、草酸和酒石酸，加速土壤中脲酶、过氧化氢酶、酸性磷酸酶和蔗糖酶的活性。上述结果表明，微生物的添加不仅促进了蓖麻幼苗的生长，提高了蓖麻对铜的耐受性，而且加强了铜在蓖麻体内的积累。