The role of topical antioxidants (AOs) on visible light plus ultraviolet A1 (VL+UVA1)-induced skin changes were evaluated. Twenty subjects with skin phototypes (SPTs) I-VI had placebo and concentrations of an AO blend applied to their back (AO 0.5%, 1.0% and 2.0%). Treated and control sites were irradiated with VL+UVA1. Colorimetric and diffuse reflectance spectroscopy (DRS) assessments were performed immediately, 24 h and 7 days after irradiation. Subjects with SPT I-III had erythema that faded within 24 h, while SPT IV-VI had persistent pigmentation. SPT I-III demonstrated significantly less erythema at the 2% AO site while SPT IV-VI demonstrated significantly less immediate pigmentation at 2% AO site and less pigmentation (approaching significance, P = 0.07) on day 7 compared with control. Immunohistochemistry from biopsies of 2% AO and placebo at 24 h did not demonstrate a significant change in COX-2 or MART-1 for any SPT. There was a decrease in cyclin D1 for SPT IV-VI which was approaching significance (P = 0.06) but not for SPT I-III. The results indicate that topical AO inhibits erythema in SPT I-III and reduces pigmentation in SPT IV-VI caused by VL+UVA1. AO may help prevent worsening of pigmentary disorders and should be incorporated into photoprotection.

中文翻译：

---

用局部抗氧化剂减轻可见光和长波 UVA1 引起的影响

评估了局部抗氧化剂 (AOs) 对可见光加紫外线 A1 (VL+UVA1) 诱导的皮肤变化的作用。20 名皮肤光型 (SPT) I-VI 的受试者在背部使用安慰剂和浓度为 AO 的混合物（AO 0.5%、1.0% 和 2.0%）。用 VL+UVA1 照射治疗部位和对照部位。在照射后 24 小时和 7 天立即进行比色和漫反射光谱 (DRS) 评估。SPT I-III 受试者的红斑在 24 小时内消退，而 SPT IV-VI 则有持续的色素沉着。SPT I-III 在 2% AO 部位的红斑显着减少，而 SPT IV-VI 在 2% AO 部位的即刻色素沉着显着减少，色素沉着减少（接近显着性，P = 0.07) 在第 7 天与对照相比。2% AO 和安慰剂在 24 小时活检的免疫组织化学未显示任何 SPT 的 COX-2 或 MART-1 的显着变化。SPT IV-VI 的 cyclin D1 下降接近显着性 ( P  = 0.06)，但 SPT I-III 没有。结果表明，局部 AO 可抑制 SPT I-III 中的红斑，并减少由 VL+UVA1 引起的 SPT IV-VI 中的色素沉着。AO 可能有助于防止色素紊乱的恶化，应纳入光保护。