In the present study, we have explored the potential of the RNAi mediated silencing of genes encoding peroxisomal biogenesis factor and β-1,3-glucanosyltransferase in Fusarium oxysporum f. sp. lycopersici (Fol) to confer resistance to Fusarium wilt in transgenic tomato plants. The partial gene fragments from these genes were utilized independently to generate hairpin RNAi constructs in appropriate silencing vectors and used for Agrobacterium-mediated transformation of tomato. The presence of gene-specific siRNAs was confirmed by stem-loop RT-PCR analysis of selected transgenic tomato lines. Transgenic lines expressing gene-specific dsRNA displayed enhanced resistance to Fol with delayed development of disease symptoms. The survival rate of transgenic tomato lines after fungal infection was higher as compared to that of the untransformed tomato plants.

在本研究中，我们探索了在尖孢镰刀菌中 RNAi 介导的编码过氧化物酶体生物发生因子和 β-1,3-葡聚糖基转移酶的基因沉默的潜力。sp。lyco persici (Fol)赋予转基因番茄植物对枯萎病的抗性。来自这些基因的部分基因片段被独立地用于在适当的沉默载体中生成发夹 RNAi 构建体，并用于农杆菌介导的番茄转化。通过对选定的转基因番茄系进行茎环 RT-PCR 分析，证实了基因特异性 siRNA 的存在。表达基因特异性 dsRNA 的转基因株系表现出对Fol的增强抗性随着疾病症状的延迟发展。与未转化的番茄植株相比，真菌感染后转基因番茄株系的存活率更高。