Plants interact with a great variety of microorganisms that inhabit the rhizosphere playing critical roles in several aspects of plant growth and protection against abiotic and biotic diseases. In this study, we performed a screening of bacteria associated with the rhizosphere of Prunus domestica trees to identify bacterial strains with plant growth-promoting activity. Ten strains isolated from the rhizosphere of P. domestica showed multiple in vitro plant growth promoting rhizobacteria (PGPR) activity such as the production of indole acetic acid, hydrogen cyanide, ammonia, solubilization of phosphates and antifungal activity against Verticillium dalhiae and Fusarium oxysporum f.sp. melonis. In planta, they significantly increased the growth (stem length, number of leaflets, leaf area and root weight) and biochemical (nitrate reductase activity, proline and chlorophyll content) parameters of tomato, as well as the rate of seed germination. Two selected strains (Pr7 and Pr8) with higher antagonistic activity against V. dalhiae and F. oxysporum f.sp. melonis protected tomato plants against Verticillium wilt and salt stress. In addition, they enhanced acclimatization of Vitis vinifera cv. Pinot noir and the peach root stock GF305 from in vitro to the greenhouse. 16S rRNA sequencing identified strains Pr7 and Pr8 as Pseudomonas stutzeri and Bacillus toyonensis, respectively. Since these two PGPR inoculants exhibited multiple traits beneficial to the examined host plants, they may be applied in the development of safe, and effective seed treatments as an alternative to chemical fungicides and fertilization but also for successful acclimatization of micropropagated plants.

植物与栖息在根际的各种微生物相互作用，在植物生长和防止非生物和生物疾病的几个方面发挥关键作用。在这项研究中，我们对与家李树根际相关的细菌进行了筛选，以确定具有促进植物生长活性的细菌菌株。10株从根际分离P.家蝇呈多体外植物生长促进根际细菌（PGPR）活性等的生产吲哚乙酸，氰化氢，氨，磷酸盐和抗真菌活性的溶解的黄萎病dalhiae和尖孢镰刀菌F。 sp.甜瓜。在植物中，它们显着增加了番茄的生长（茎长、小叶数量、叶面积和根重）和生化（硝酸还原酶活性、脯氨酸和叶绿素含量）参数，以及种子发芽率。两个所选择的应变（PR7和PR8）用针对更高拮抗活性V. dalhiae和尖孢镰孢病菌 melonis保护番茄植物免受黄萎病和盐胁迫。此外，它们增强了Vitis vinifera cv. 的驯化。从体外到温室的黑比诺和桃砧木GF305 。16S rRNA 测序确定菌株 Pr7 和 Pr8 为施氏假单胞菌和分别为Bacillus toyonensis。由于这两种 PGPR 接种剂表现出对受检宿主植物有益的多种性状，因此它们可用于开发安全、有效的种子处理，作为化学杀菌剂和施肥的替代品，也可用于微繁植物的成功驯化。