Enterococcus faecium ST20Kc and ST41Kc were isolated from kimchi, a traditional Korean fermented cabbage. Bacteriocins produced by both strains exhibited strong activity against Listeria monocytogenes and various Enterococcus spp., including 30 vancomycin-resistant enterococcal strains, but not against other lactic acid bacteria (LAB) on the evaluated test panel. The antimicrobials produced by the strains were found to be proteinaceous and stable even after exposure to varying pH, temperature, and chemicals used in the industry and laboratory processes. Antimicrobial activity of both strains was evaluated as bactericidal against exponentially growing cultures of L. monocytogenes ATCC® 15313™ and Enterococcus faecalis 200A. Based on tricine-SDS-PAGE, the molecular weights of the bacteriocins produced by the strains were between 4 and 6 kDa. Additionally, both strains were susceptible to antibiotics, including vancomycin, kanamycin, gentamycin, ampicillin, streptomycin, tylosin, chloramphenicol, clindamycin, and tetracycline. Adhesion genes, map, mub, and EF-Tu, were also detected in the genomes of both strains. With gastrointestinal stress induction, both strains showed high individual survival rates, and capability to reduce viable counts of L. monocytogenes ATCC® 15313™ and Enterococcus faecalis 200A in mixed cultures. Based on the metabolomics analysis, both strains were found to produce additional antimicrobial compounds, particularly, lactic acid, phenyllactic acid, and phenethylamine, which can be potentially involved in the antimicrobial interaction with pathogenic microorganisms.

屎肠球菌ST20Kc 和 ST41Kc 是从韩国传统发酵卷心菜泡菜中分离出来的。两种菌株产生的细菌素对单核细胞增生李斯特菌和各种肠球菌，包括 30 种耐万古霉素肠球菌菌株均表现出很强的活性，但对评估的测试面板上的其他乳酸菌 (LAB) 没有表现出很强的活性。发现这些菌株产生的抗微生物剂即使在暴露于工业和实验室过程中使用的不同 pH 值、温度和化学品后仍具有蛋白质性质和稳定性。两种菌株的抗菌活性被评估为对单核细胞增生李斯特菌ATCC® 15313™ 和粪肠球菌的指数生长培养物的杀菌作用200A。基于tricine-SDS-PAGE，菌株产生的细菌素的分子量在4-6kDa之间。此外，这两种菌株对抗生素敏感，包括万古霉素、卡那霉素、庆大霉素、氨苄青霉素、链霉素、泰乐菌素、氯霉素、克林霉素和四环素。在两种菌株的基因组中也检测到粘附基因map、mub和EF-Tu。通过胃肠道应激诱导，两种菌株均显示出较高的个体存活率，并能够减少单核细胞增生李斯特氏菌ATCC® 15313™ 和粪肠球菌的活菌数200A 混合培养。根据代谢组学分析，发现这两种菌株都能产生额外的抗菌化合物，特别是乳酸、苯乳酸和苯乙胺，它们可能参与与病原微生物的抗菌相互作用。