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S-Adenosyl-L-Homocysteine Hydrolase Immobilized on Citric Acid-capped Gallium Oxyhydroxide on SWCNTs Modified Electrode for AdoHcy Impedimetric Sensing
Electroanalysis ( IF 3 ) Pub Date : 2021-09-21 , DOI: 10.1002/elan.202100362
Sara Knežević 1 , Milos Ognjanović 2 , Marija Gavrović Jankulović 1 , Tatjana Đurašinović 3 , Bratislav Antić 4 , Sanja Vranješ Đurić 5 , Dalibor Stankovic 6
Affiliation  

S-Adenosyl-L-Homocysteine (AdoHcy) is a crucial pathological factor in homocysteine-associated disorders. We developed an impedimetric approach for single drop S-Adenosyl-L-Homocysteine detection, based on S-Adenosyl-L-Homocysteine hydrolase (SAH), produced in the prokaryotic expression system (E. coli) by recombinant DNA technology, immobilized on citrate acid-coated gallium oxyhydroxide dispersed over single-walled carbon nanotubes (CA/GaO(OH)@SWCNT). The proposed biosensor offers detection of AdoHcy, with a limit of detection (LOD) of 0.17 μM. with operating linear range from 1 to 44 μM. The remarkable stability of gallium oxyhydroxide crystal was further improved by citric acid coating and its optical, electrical and physical anisotropy was skipped using SWCNTs.

中文翻译:

S-腺苷-L-同型半胱氨酸水解酶固定在柠檬酸封端的氢氧化镓上的 SWCNTs 修饰电极上用于 AdoHcy 阻抗传感

S-腺苷-L-同型半胱氨酸 (AdoHcy) 是同型半胱氨酸相关疾病的关键病理因素。我们开发了一种单滴 S-腺苷-L-同型半胱氨酸检测方法,该方法基于 S-腺苷-L-同型半胱氨酸水解酶 (SAH),通过重组 DNA 技术在原核表达系统(大肠杆菌)中产生,固定在柠檬酸盐上分散在单壁碳纳米管上的酸涂层羟基氧化镓 (CA/GaO(OH)@SWCNT)。所提出的生物传感器可检测 AdoHcy,检测限 (LOD) 为 0.17 μM。工作线性范围为 1 至 44 μM。柠檬酸涂层进一步提高了羟基氧化镓晶体的显着稳定性,并且使用单壁碳纳米管跳过了其光学、电学和物理各向异性。
更新日期:2021-09-21
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