Herein, we propose an element probe based CRISPR/Cas14 detection platform and apply it to the detection of non-nucleic-acid targets. Combining metal isotope detection and CRISPR/Cas14 biosensing, the sensitive detection of non-nucleic-acid targets could be realized. We designed and optimized the element probe, which proved that Cas14 has a preference for longer lengths in element probe cleavage. Using this method, the quantitative detection of trace aqueous ampicillin can be achieved within 45 minutes at room temperature (25 °C). A detection limit as low as 2.06 nM is obtained with excellent performance in anti-interference tests and complex matrix detection.

中文翻译：

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用于非核酸靶标的基于元素探针的 CRISPR/Cas14 生物测定

在此，我们提出了一种基于元素探针的 CRISPR/Cas14 检测平台，并将其应用于非核​​酸靶点的检测。结合金属同位素检测和CRISPR/Cas14生物传感，可实现非核酸靶点的灵敏检测。我们设计并优化了元素探针，这证明了 Cas14 在元素探针切割中更喜欢更长的长度。使用该方法，可在室温（25°C）下45分钟内实现痕量氨苄青霉素水溶液的定量检测。检测限低至 2.06 nM，在抗干扰测试和复杂基质检测中表现出色。