Traditional hydrogels often fail to match the dynamic interactions between mechanical and cellular behaviors exhibited by the natural cartilage extracellular matrix. In this research, we constructed a novel hybrid hydrogels system based on sodium alginate and polyglutamic acid. By controlling the grafting rate and concentration of polymer, the gelation time and mechanical strength can be adjusted between range of 8–28 s and 60–144 kPa. By adding microcrystalline cellulose into the system, so that the degradation time was prolonged (125%) and the swelling rate was reduced (470%). Additionally, the presence of hydrazone bonds gives the system some dynamic response characteristics, and the hydrogel exhibits excellent self healing and injectable ability. It was found that the system had positive cytocompatibility (80%), which accelerated regulatory gene expression in cartilage tissue. In conclusion, this injectable hydrogel with self-healing and customizable mechanical strength will have broad application prospects in future biomedical engineering.

传统的水凝胶通常无法匹配天然软骨细胞外基质所表现出的机械和细胞行为之间的动态相互作用。在这项研究中，我们构建了一种基于海藻酸钠和聚谷氨酸的新型混合水凝胶系统。通过控制聚合物的接枝率和浓度，胶凝时间和机械强度可以在 8-28 s 和 60-144 kPa 之间调节。通过向体系中加入微晶纤维素，使降解时间延长（125%），溶胀率降低（470%）。此外，腙键的存在赋予了系统一些动态响应特性，水凝胶表现出优异的自愈和可注射能力。发现该系统具有阳性细胞相容性（80%），这加速了软骨组织中的调节基因表达。综上所述，这种具有自愈性和可定制机械强度的可注射水凝胶将在未来的生物医学工程中具有广阔的应用前景。