Exogenous mold infection of bamboo-based material results in the reduction of bamboo durability. Thymol, a remarkably safe and green fungicide, can be successfully applied on bamboo to resist mold attacks. The leachability of volatile thymol in bamboo led to a decrease in bamboo anti-mold activity due to the lack of chemical bond attachment. In this study, a novel laccase-mediated system was used to improve bamboo anti-mold activity by enhancing the fixation of thymol. The results showed that this modification considerably restricted the rate of mold infection. The volatility of thymol in modified bamboo decreased from 0.10 % to 0.05 %, which extended the activity persistence of mold resistance. Meanwhile, the surface hydrophobicity of the modified bamboo increased from 45.3 ° to 118.6 °. Among the three major components of bamboo investigated, only lignin experienced a grafting reaction with thymol catalyzed by laccase. The ether bond was identified as the major linkage between lignin and thymol in laccase-mediated thymol-modified bamboo via multiple characterization methods. This work not only demonstrates a feasible avenue for bamboo preservation, but also elucidates the inter- and intra-chemical bonds in bamboo after laccase-mediated thymol modification.

竹基材料的外源霉菌感染导致竹材耐久性降低。百里酚是一种非常安全且绿色的杀菌剂，可成功应用于竹子以抵抗霉菌侵袭。由于缺乏化学键附着，竹子中挥发性百里酚的可浸出性导致竹子抗霉活性降低。在这项研究中，一种新的漆酶介导系统用于通过增强百里酚的固定来提高竹子的抗霉菌活性。结果表明，这种修改极大地限制了霉菌感染的速度。改性竹百里酚挥发度从0.10%降低到0.05%，延长了抗霉活性的持续性。同时，改性竹材的表面疏水性从45.3°增加到118.6°。在研究的竹子三大成分中，只有木质素在漆酶催化下与百里酚发生接枝反应。通过多种表征方法，醚键被确定为漆酶介导的百里酚改性竹中木质素和百里酚之间的主要连接。这项工作不仅证明了竹子保存的可行途径，而且阐明了漆酶介导的百里酚修饰后竹子中的化学间和化学内键。