Mercury (Hg) causes serious health issues in its all forms. Deficiency as well as excess of copper ion (Cu²⁺) in human body is hazardous. A series of four compounds have been derived from carboxylated benzoic acids (benzoic acid, isophthalic acid, terephthalic acid and phthalic acid) and 4H-1,2,4 triazole-4-amine and characterized. Fluorescence detection of Hg²⁺ was recorded by the derivates with benzoic acid and isophthalic acid while the derivatives of terephthalic acid and phthalic acid detect Cu²⁺ by fluorescence “off” mode. Metal ions like Li⁺, Na⁺, K⁺, Zn²⁺, Al³⁺, Mg²⁺, Mn²⁺, Co²⁺, Ni²⁺, Cu²⁺, Cd²⁺, Pb²⁺ and Hg²⁺ found not to interfere. The stoichiometry of binding is 1:1 for the benzoic acid derivative with Hg²⁺ while it is 1:2 for the other three derivatives. The binding constants are ca. 10^–4.5 between the sensors and Hg²⁺ or Cu²⁺ and detection limits are around 10^–5.5 M. DFT calculation provided optimized geometries of the sensors and confirmed the stoichiometry of binding with Hg²⁺/Cu²⁺.

汞 (Hg) 会导致各种形式的严重健康问题。人体中铜离子（Cu ²⁺ ）的缺乏和过量都是危险的。从羧基苯甲酸（苯甲酸、间苯二甲酸、对苯二甲酸和邻苯二甲酸）和4H-1,2,4三唑-4-胺中衍生出一系列四种化合物并进行了表征。Hg ²⁺的荧光检测由苯甲酸和间苯二甲酸的衍生物记录，而对苯二甲酸和邻苯二甲酸的衍生物通过荧光“关闭”模式检测Cu ^{2+ 。}Li ⁺、Na ⁺、K ⁺、Zn ²⁺、Al ³⁺、Mg ²⁺、Mn ^{2+等金属离子}、Co ²⁺、Ni ²⁺、Cu ²⁺、Cd ²⁺、Pb ²⁺和Hg ²⁺没有发现干扰。苯甲酸衍生物与 Hg ²⁺的结合化学计量比为 1:1，而其他三种衍生物的结合化学计量比为 1:2。绑定常数约为。传感器与 Hg ²⁺或 Cu ²⁺之间的误差为10 ^–4.5，检测限约为 10 ^{–5.5 M。DFT计算提供了传感器的优化几何形状，并证实了与 Hg 2+} /Cu ²⁺ 结合的化学计量。