This study aimed to assess the tissue content of essential and toxic metals including lead (Pb), cadmium (Cd), arsenic (As), silver (Ag), aluminum (Al), chromium (Cr), copper (Cu), iron (Fe), selenium (Se), and zinc (Zn) in the breast cancerous tissues compared to the non-cancerous tissue. The biopsy specimens of 63 breast cancers along with 63 adjacent healthy tissues in Kurdistan Province, Iran, were collected from 2019 to 2020 and assayed using ICP-MS (Agilent 7900). The results of the Mann-Whitney test illustrated that the concentration of Pb, Cd, As, Cr, Cu, and Se were significantly elevated in cancerous tissue (p < 0.05), while Zn was the only trace element with higher levels in healthy subjects (p < 0.05). Moreover, weak to moderate correlations between elements were observed in the cancerous group including Al-Cr (r=0.60), As-Cu (r=0.52), and Cu-Se (r=0.56). In contrast, no correlation over 0.50 was found between trace elements in the non-cancerous group. Raw risk differences (RDs) accounted for a significant effect for Pb, Cd, As, Ag, Cr, Se, and Zn on the development of breast cancer. In conclusion, elevated levels of As, Cd, Cu, Pb, and Se may contribute to enhancing the risk of breast cancer.

本研究旨在评估组织中必需和有毒金属的含量，包括铅 (Pb)、镉 (Cd)、砷 (As)、银 (Ag)、铝 (Al)、铬 (Cr)、铜 (Cu)、铁与非癌组织相比，乳腺癌组织中的 (Fe)、硒 (Se) 和锌 (Zn)。从 2019 年到 2020 年收集了伊朗库尔德斯坦省 63 例乳腺癌和 63 例邻近健康组织的活检标本，并使用 ICP-MS（安捷伦 7900）进行了分析。Mann-Whitney 检验结果表明，Pb、Cd、As、Cr、Cu 和 Se 的浓度在癌组织中显着升高 ( p < 0.05)，而 Zn 是健康受试者中唯一含量较高的微量元素( p< 0.05)。此外，在包括 Al-Cr ( r =0.60)、As-Cu ( r =0.52) 和 Cu-Se ( r =0.56)的癌变组中观察到元素之间的弱到中等相关性。相反，未发现非癌组微量元素之间的相关性超过 0.50。原始风险差异 (RD) 解释了 Pb、Cd、As、Ag、Cr、Se 和 Zn 对乳腺癌发展的显着影响。总之，升高的 As、Cd、Cu、Pb 和 Se 水平可能会增加患乳腺癌的风险。