Nanotechnology is drawing attention nowadays due to its ability to regulate metals into nanosize, ultimately changing metal's physical, chemical, and optical properties. Silver nanoparticles are known for their potential impact as antimicrobial agents due to their inherent property penetrating the cell wall. The present study aimed to develop and statistically optimise using a novel combination of capsaicin loaded silver nanoparticles (AgCNPs) as an effective anti-bacterial agent to treat psoriasis using a green approach. Ascorbic acid was used as a reducing agent to fabricate silver nanoparticles. The formulation parameters optimisation was conducted using Box-Behnken Design (3×3 factorial design). The loading of capsaicin was confirmed by attenuated total reflectance-fourier transform infrared spectroscopy. Energy-dispersive X-ray spectroscopy-scanning electron microscopy (EDX-SEM) confirmed the existence of silver; net-like structure revealed in SEM and high-resolution transmission electron microscopy further confirmed the nano size of the formulation. Differential scanning calorimetry and X-ray diffraction demonstrated the capsaicin transformed into amorphous after encapsulated. An in-vitro microbial study showed that the 0.10 M formulation of AgCNPs exerted potent anti-bacterial activity, which can be considered an alternative anti-bacterial agent. It also displayed that the zone of inhibition was significantly high in gram-negative bacteria (E. coli) than gram-positive bacteria (S. aureus). Green synthesised AgCNPs showed highly significant anti-bacterial activity, which indicates that this formulation can be very promising for treating psoriasis.

中文翻译：

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使用绿色方法合成负载辣椒素的银纳米颗粒及其对人类病原体的抗菌活性。

由于纳米技术能够将金属调节为纳米尺寸，最终改变金属的物理、化学和光学特性，因此纳米技术如今备受关注。银纳米粒子因其固有的穿透细胞壁的特性而作为抗菌剂的潜在影响而闻名。本研究旨在开发和统计优化使用负载辣椒素的银纳米粒子 (AgCNP) 作为有效抗菌剂的新型组合，以使用绿色方法治疗银屑病。抗坏血酸被用作还原剂来制造银纳米颗粒。配方参数优化使用 Box-Behnken 设计（3×3 因子设计）进行。通过衰减全反射-傅立叶变换红外光谱证实了辣椒素的负载。能量色散 X 射线光谱扫描电子显微镜 (EDX-SEM) 证实了银的存在；SEM 和高分辨率透射电子显微镜中显示的网状结构进一步证实了该配方的纳米尺寸。差示扫描量热法和 X 射线衍射表明辣椒素在封装后转化为无定形。一个体外微生物研究表明，0.10 M 的 AgCNPs 配方具有强大的抗菌活性，可被视为一种替代抗菌剂。它还表明，革兰氏阴性菌（大肠杆菌）的抑菌圈明显高于革兰氏阳性菌（金黄色葡萄球菌）。Green 合成的 AgCNPs 显示出非常显着的抗菌活性，这表明该制剂在治疗银屑病方面非常有前景。