For craniofacial bone regeneration, how to promote vascularized bone regeneration is still a significant problem, and the controlled release of trace elements vital to osteogenesis has attracted attention. In this study, an ion co-delivery system was developed to promote angiogenesis and osteogenesis. Magnesium ions (Mg²⁺) and lanthanum ions (La³⁺) were selected as biosignal molecules because Mg²⁺ can promote angiogenesis and both of them can enhance bone formation. Microspheres made of poly(lactide-co-glycolide) were applied to load La₂(CO₃)₃, which was embedded into a MgO/MgCO₃-loaded cryogel made of photocrosslinkable gelatin methacryloyl to enable co-delivery of Mg²⁺ and La³⁺. Evaluations of angiogenesis and osteogenesis were conducted via both in vitro cell culture using human bone marrow mesenchymal stromal cells and in vivo implantation using a rat model with calvarial defect (5 mm in diameter). Compared to systems releasing only Mg²⁺ or La³⁺, the combination system demonstrated more significant effects on blood vessels formation, thereby promoting the regeneration of vascularized bone tissue. At 8 weeks post-implantation, the new bone volume/total bone volume ratio reached a value of 40.1 0.9%. In summary, a properly designed scaffold system with the capacity to release ions of different bioactivities in a desired pattern can be a promising strategy to meet vascularized bone regeneration requirements.

对于颅面骨再生，如何促进血管化骨再生仍然是一个重大问题，而对成骨至关重要的微量元素的控释受到关注。在这项研究中，开发了一种离子共传递系统来促进血管生成和成骨。选择镁离子（Mg ²⁺）和镧离子（La ³⁺）作为生物信号分子是因为Mg ²⁺可以促进血管生成，并且它们都可以促进骨形成。由聚（丙交酯-共-乙交酯）制成的微球用于负载 La ₂ (CO ₃ ) ₃，将其嵌入到 MgO/MgCO _3中^{由可光交联的明胶甲基丙烯酰基制成的负载冷冻凝胶，以实现 Mg 2+}和 La ³⁺的共同递送。通过使用人骨髓间充质基质细胞的体外细胞培养和使用颅骨缺损（直径 5 mm）的大鼠模型进行体内植入，对血管生成和成骨进行了评估。与仅释放 Mg ²⁺或 La ^{3+的系统相比}，该组合系统对血管形成表现出更显着的影响，从而促进血管化骨组织的再生。植入后8周，新骨量/总骨量比值达到40.1±0.9%。总之，具有以所需模式释放不同生物活性离子的能力的适当设计的支架系统可能是满足血管化骨再生要求的有希望的策略。