This work reports the association of poly(styrene-co-acrylic acid) random copolymer, P(S-r-AA), with poly(vinylidene fluoride) (PVDF) in the fabrication of electrospun fibers for blood filtration. At first, effort was put into the determination of the styrene/acrylic acid molar ratio in P(S-r-AA) copolymer that would improve best the wetting of nanofibers. With a 19/81 molar ratio, the water contact angle of the modified membrane (M5) was 127 ± 1°, while it was slightly higher for the unmodified membrane (M0, 136 ± 1°). However, the copolymer significantly improved hydration after prolonged contact of the membrane with an aqueous medium, as the swelling ratio increased dramatically from 19% (M0) to 1600% (M5). Nanofibers were all found to be physically and chemically homogeneous, with a pore size increasing from 0.56 μm to 1.25 μm for M0 and M5, respectively, and a regular copolymer distribution on the surface. While no flow could be recorded through M0 with white blood cells (WBCs) concentrate, 10-fold diluted whole blood or whole blood, permeation and separation were achievable with M5. 100%, 100% and 99.99% retention of leukocytes was measured with WBCs concentrate, 10-fold diluted whole blood, and pure whole blood using this membrane. Besides, it allowed to recover 79% of red blood cells. Although effort is still needed to enhance the nanofibers biocompatibility and the RBCs recovery, the results of this study prove that PVDF-based nanofibers have potential to be efficient separation media of whole blood.

这项工作报告聚协会（苯乙烯-共-丙烯酸）无规共聚物，P（S- [R在用于血液过滤电纺丝纤维的制造-AA），与聚（偏二氟乙烯）（PVDF）。首先，努力确定 P(S- r) 中苯乙烯/丙烯酸的摩尔比-AA) 共聚物，可以最好地改善纳米纤维的润湿性。摩尔比为 19/81 时，改性膜的水接触角 (M5) 为 127 ± 1°，而未改性膜的水接触角略高 (M0, 136 ± 1°)。然而，在膜与水性介质长时间接触后，共聚物显着改善了水合作用，因为溶胀率从 19% (M0) 急剧增加到 1600% (M5)。发现纳米纤维在物理和化学上都是均匀的，M0 和 M5 的孔径分别从 0.56 μm 增加到 1.25 μm，并且表面上有规则的共聚物分布。虽然使用白细胞 (WBC) 浓缩物无法通过 M0 记录流量，但使用 M5 可以实现 10 倍稀释的全血或全血、渗透和分离。100%、100% 和 99。99% 的白细胞保留是用 WBC 浓缩液、10 倍稀释的全血和使用这种膜的纯全血测量的。此外，它可以回收 79% 的红细胞。尽管仍需要努力提高纳米纤维的生物相容性和红细胞回收率，但本研究的结果证明基于 PVDF 的纳米纤维具有成为全血高效分离介质的潜力。